Browsing Bergen Open Research Archive by Author "Halskau, Øyvind"

Now showing items 1-20 of 20

    • The Arabidopsis (ASHH2) CW domain binds monomethylated K4 of the histone H3 tail through conformational selection 

      Dobrovolska, Olena; Brilkov, Maxim; Madeleine, Noëlly; Ødegaard-Fougner, Øyvind; Strømland, Øyvind; Martin, Stephen R.; De Marco, Valeria; Christodoulou, Evangelos; Teigen, Knut; Isaksson, Johan; Underhaug, Jarl; Reuter, Nathalie; Aalen, Reidunn B.; Aasland, Rein; Halskau, Øyvind (Journal article; Peer reviewed, 2020)
      Chromatin post‐translational modifications are thought to be important for epigenetic effects on gene expression. Methylation of histone N‐terminal tail lysine residues constitutes one of many such modifications, executed ...

    • Binding Specificity of ASHH2 CW Domain Toward H3K4me1 Ligand Is Coupled to Its Structural Stability Through Its α1-Helix 

      Bril'kov, Maxim; Dobrovolska, Olena; Ødegård-Fougner, Øyvind; Turcu, Diana Cornelia; Strømland, Øyvind; Underhaug, Jarl; Aasland, Rein; Halskau, Øyvind (Journal article; Peer reviewed, 2022-04-13)
      The CW domain binds to histone tail modifications found in different protein families involved in epigenetic regulation and chromatin remodeling. CW domains recognize the methylation state of the fourth lysine on histone ...

    • Bioactive Metabolites of Marine Origin Have Unusual Effects on Model Membrane Systems 

      Jakubec, Martin; Totland, Christian; Rise, Frode; Chamgordani, Elahe Jafari; Paulsen, Britt; Maes, Louis; Matheeussen, An; Gundersen, Lise-Lotte; Halskau, Øyvind (Journal article; Peer reviewed, 2020)
      Marine sponges and soft corals have yielded novel compounds with antineoplastic and antimicrobial activities. Their mechanisms of action are poorly understood, and in most cases, little relevant experimental evidence is ...

    • The biosynthesis of phospholipids is linked to the cell cycle in a model eukaryote 

      Vitova, Milada; Lanta, Vojtech; Cizkova, Maria; Jakubec, Martin; Rise, Frode; Halskau, Øyvind; Bisova, Katerina; Furse, Samuel Robert (Journal article; Peer reviewed, 2021)
      The structural challenges faced by eukaryotic cells through the cell cycle are key for understanding cell viability and proliferation. We tested the hypothesis that the biosynthesis of structural lipids is linked to the ...

    • Cholesterol-containing lipid nanodiscs promote an α-synuclein binding mode that accelerates oligomerization 

      Jakubec, Martin; Bariås, Espen; Furse, Samuel Robert; Govasli Larsen, Morten Andreas; George, Vinnit; Turcu, Diana Cornelia; Iashchishyn, Igor A.; Morozova-Roche, Ludmilla A.; Halskau, Øyvind (Journal article; Peer reviewed, 2021)
      Dysregulation of the biosynthesis of cholesterol and other lipids has been implicated in many neurological diseases, including Parkinson's disease. Misfolding of α-synuclein (α-Syn), the main actor in Parkinson's disease, ...

    • Contrasting the phospholipid profiles of two neoplastic cell lines reveal a high PC:PE ratio for SH-SY5Y cells relative to A431 cells 

      Bariås, Espen; Jakubec, Martin; Førsund, Elise; Hjørnevik, Linda Veka; Lewis, Aurelia Eva; Halskau, Øyvind (Journal article; Peer reviewed, 2023)
      Lipids have been implicated in Parkinson's Disease (PD). We therefore studied the lipid profile of the neuroblastoma SH-SY5Y cell line, which is used extensively in PD research and compared it to that of the A431 epithelial ...

    • Detection of misfolded protein aggregates from a clinical perspective 

      Jakubec, Martin; Strømland, Øyvind; Furse, Samuel Robert; Halskau, Øyvind (Peer reviewed; Journal article, 2016)
      Neurodegenerative Protein Misfolding Diseases (PMDs), such as Alzheimer’s (AD), Parkinson’s (PD) and prion diseases, are generally difficult to diagnose before irreversible damage to the central nervous system damage has ...

    • Evidence that Listeria innocua modulates its membrane’s stored curvature elastic stress, but not fluidity, through the cell cycle 

      Furse, Samuel Robert; Jakubec, Martin; Rise, Frode; Williams, Huw E.; Rees, Catherine E.D.; Halskau, Øyvind (Peer reviewed; Journal article, 2017-08-14)
      This paper reports that the abundances of endogenous cardiolipin and phosphatidylethanolamine halve during elongation of the Gram-positive bacterium Listeria innocua. The lyotropic phase behaviour of model lipid systems ...

    • Fast and Quantitative Phospholipidomic Analysis of SH-SY5Y Neuroblastoma Cell Cultures Using LC-MS/MS and 31P NMR 

      Jakubec, Martin; Bariås, Espen; Kryuchkov, Fedor; Hjørnevik, Linda Veka; Halskau, Øyvind (Peer reviewed; Journal article, 2019)
      Global lipid analysis still lags behind proteomics with respect to the availability of databases, experimental protocols, and specialized software. Determining the lipidome of cellular model systems in common use is of ...

    • HAMLET forms annular oligomers when deposited with phoshpolipid monolayers 

      Baumann, Anne; Gjerde, Anja Underhaug; Ying, Ming; Svanborg, Catharina; Holmsen, Holm A.; Glomm, Wilhelm; Martinez, Aurora; Halskau, Øyvind (Peer reviewed; Journal article, 2012-04)
      Recently, the anticancer activity of human α-lactalbumin made lethal to tumor cells (HAMLET) has been linked to its increased membrane affinity in vitro, at neutral pH, and ability to cause leakage relative to the inactive ...

    • HAMLET interacts with lipid membranes and perturbs their structure and integrity 

      Mossberg, Ann-Kristin; Puchades, Maja; Halskau, Øyvind; Baumann, Anne; Lanekoff, Ingela; Chao, Yinxia; Martinez, Aurora; Svanborg, Catharina; Karlsson, Roger (Peer reviewed; Journal article, 2010-01-23)
      Background Cell membrane interactions rely on lipid bilayer constituents and molecules inserted within the membrane, including specific receptors. HAMLET (human α-lactalbumin made lethal to tumor cells) is a tumoricidal ...

    • How Honey Bee Vitellogenin Holds Lipid Cargo: A Role for the C-Terminal 

      Leipart, Vilde; Halskau, Øyvind; Amdam, Gro Vang (Journal article; Peer reviewed, 2022-06-09)
      Vitellogenin (Vg) is a phylogenetically broad glycolipophosphoprotein. A major function of this protein is holding lipid cargo for storage and transportation. Vg has been extensively studied in honey bees (Apis mellifera) ...

    • Investigating the Disordered and Membrane-Active Peptide A-Cage-C Using Conformational Ensembles 

      Dobrovolska, Olena; Strømland, Øyvind; Handegård, Ørjan Sele; Jakubec, Martin; Govasli Larsen, Morten Andreas; Skjevik, Åge Aleksander; Frøystein, Nils Åge; Teigen, Knut; Halskau, Øyvind (Journal article; Peer reviewed, 2021)
      The driving forces and conformational pathways leading to amphitropic protein-membrane binding and in some cases also to protein misfolding and aggregation is the subject of intensive research. In this study, a chimeric ...

    • The N-terminal sequence of tyrosine hydroxylase is a conformationally versatile motif that binds 14-3-3 proteins and membranes 

      Skjevik, Åge Aleksander; Mileni, Mauro; Baumann, Anne; Halskau, Øyvind; Teigen, Knut; Raymond C., Stevens; Martinez, Aurora (Peer reviewed; Journal article, 2014-01-09)
      Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of catecholamine neurotransmitters, and a reduction in TH activity is associated with several neurological diseases. Human TH is regulated, among ...

    • The Peripheral Binding of 14-3-3γ to Membranes Involves Isoform-Specific Histidine Residues 

      Bustad, Helene J.; Skjærven, Lars; Ying, Ming; Halskau, Øyvind; Baumann, Anne; Rodriguez-Larrea, David; Costas, Miguel; Underhaug, Jarl; Sanchez-Ruiz, Jose M.; Martinez, Aurora (Peer reviewed; Journal article, 2012-11-26)
      Mammalian 14-3-3 protein scaffolds include seven conserved isoforms that bind numerous phosphorylated protein partners and regulate many cellular processes. Some 14-3-3-isoforms, notably γ, have elevated affinity for ...

    • Plasma-derived exosome-like vesicles are enriched in lyso-phospholipids and pass the blood-brain barrier 

      Jakubec, Martin; Maple-Grødem, Jodi; Akbari, Saleha; Nesse, Susanne; Halskau, Øyvind; Mork-Jansson, Astrid Elisabeth (Journal article; Peer reviewed, 2020)
      Exosomes are vesicles involved in intercellular communication. Their membrane structure and core content is largely dependent on the cell of origin. Exosomes have been investigated both for their biological roles and their ...

    • Resolving the zinc binding capacity of honey bee vitellogenin and locating its putative binding sites 

      Leipart, Vilde; Enger, Øyvind; Turcu, Diana Cornelia; Dobrovolska, Olena; Drabløs, Finn; Halskau, Øyvind; Amdam, Gro Vang (Journal article; Peer reviewed, 2022)
      The protein vitellogenin (Vg) plays a central role in lipid transportation in most egg-laying animals. High Vg levels correlate with stress resistance and lifespan potential in honey bees (Apis mellifera). Vg is the primary ...

    • Structure prediction of honey bee vitellogenin: a multi-domain protein important for insect immunity 

      Leipart, Vilde; Montserrat Canals, Mateu; Cunha, Eva S.; Luecke, Hartmut; Herrero-Galán, Elías; Halskau, Øyvind; Amdam, Gro Vang (Journal article; Peer reviewed, 2022)
      Vitellogenin (Vg) has been implicated as a central protein in the immunity of egg-laying animals. Studies on a diverse set of species suggest that Vg supports health and longevity through binding to pathogens. Specific ...

    • Two homologous neutrophil serine proteases bind to POPC vesicles with different affinities: When aromatic amino acids matter 

      Schillinger, Anne-Sophie; Grauffel, Cédric; Khan, Hanif Muhammad; Halskau, Øyvind; Reuter, Nathalie (Peer reviewed; Journal article, 2014-12)
      Neutrophil serine proteases Proteinase 3 (PR3) and human neutrophil elastase (HNE) are homologous antibiotic serine proteases of the polymorphonuclear neutrophils. Despite sharing a 56% sequence identity they have been ...

    • α-lactalbumin: oleic acid complex spontaneously delivers oleic acid to artificial and erythrocyte membranes 

      Wen, Hanzhen; Strømland, Øyvind; Halskau, Øyvind (Peer reviewed; Journal article, 2015-08-19)
      Human α-lactalbumin made lethal to tumor cells (HAMLET) is a tumoricidal complex consisting of human α-lactalbumin and multiple oleic acids (OAs). OA has been shown to play a key role in the activity of HAMLET and its ...