Blar i Bergen Open Research Archive på forfatter "Kommedal, Øyvind"
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Bacteria and fungi in acute cholecystitis. A prospective study comparing next generation sequencing to culture
Dyrhovden, Ruben; Øvrebø, Kjell Kåre; Nordahl, Magnus Vie; Nygaard, Randi M.; Ulvestad, Elling; Kommedal, Øyvind (Peer reviewed; Journal article, 2020)Objectives: Guidelines for antibiotic treatment of acute cholecystitis are based on studies using culture techniques for microbial identification. Microbial culture has well described limitations and more comprehensive ... -
The bacterial aetiology of pleural empyema. A descriptive and comparative metagenomic study
Dyrhovden, Ruben; Nygaard, RM; Patel, R; Ulvestad, Elling; Kommedal, Øyvind (Peer reviewed; Journal article, 2019)Objectives: The view of pleural empyema as a complication of bacterial pneumonia is changing because many patients lack evidence of underlying pneumonia. To further our understanding of pathophysiological mechanisms, we ... -
The changing spectrum of microbial aetiology of respiratory tract infections in hospitalized patients before and during the COVID-19 pandemic
Serigstad, Sondre; Markussen, Dagfinn Lunde; Ritz, Christian; Ebbesen, Marit Helen; Knoop, Siri Tandberg; Kommedal, Øyvind; Heggelund, Lars; Ulvestad, Elling; Bjørneklett, Rune Oskar; Grewal, Harleen (Journal article; Peer reviewed, 2022-09-30)Background: The COVID-19 pandemic was met with strict containment measures. We hypothesized that societal infection control measures would impact the number of hospital admissions for respiratory tract infections, as well ... -
Characterization of abscesses from liver, pancreas and kidney using deep sequencing of the 16S rRNA gene
Bivand, Joanna Malgorzata; Nygaard, Randi Monsen; Kommedal, Øyvind (Journal article; Peer reviewed, 2021)To characterize the microbial communities in abscess material from liver, pancreas, and kidneys, we performed deep sequencing of the 16S rRNA gene, in addition to cultivation and Sanger based 16S rRNA gene sequencing ... -
Comparison of rapid molecular testing methods for detecting respiratory viruses in emergency care: a prospective study
Markussen, Dagfinn Lunde; Grewal, Harleen; Knoop, Siri Tandberg; Serigstad, Sondre; Kommedal, Øyvind; Ebbesen, Marit Helen; Ulvestad, Elling; Bjørneklett, Rune Oskar (Journal article; Peer reviewed, 2022)Background Respiratory tract infections (RTIs) caused by contagious viruses are common among patients presenting to the emergency department (ED). Early detection of these viruses can help prevent nosocomial transmissio ... -
The diagnostic utility of microscopic quality assessment of sputum samples in the era of rapid syndromic PCR testing
Markussen, Dagfinn Lunde; Ebbesen, Marit Helen; Serigstad, Sondre; Knoop, Siri Tandberg; Ritz, Christian; Bjørneklett, Rune Oskar; Kommedal, Øyvind; Jenum, Synne; Ulvestad, Elling; Grewal, Harleen (Journal article; Peer reviewed, 2023)This prospective study assessed the value of initial microscopy evaluation of sputum samples submitted for rapid syndromic PCR-based testing. Bacterial detections by the BioFire FilmArray Pneumonia Panel plus in 126 high- ... -
Diagnostic utility of oropharyngeal swabs as an alternative to lower respiratory tract samples for PCR-based syndromic testing in patients with community-acquired pneumonia
Serigstad, Sondre; Knoop, Siri Tandberg; Markussen, Dagfinn Lunde; Ulvestad, Elling; Bjørneklett, Rune Oskar; Ebbesen, Marit Helen; Kommedal, Øyvind; Grewal, Harleen (Journal article; Peer reviewed, 2023)Syndromic PCR-based analysis of lower respiratory tract (LRT) samples in patients with community-acquired pneumonia (CAP) improves the bacterial yield and time-to-results compared to culture-based methods. However, obtaining ... -
Escherichia marmotae—a Human Pathogen Easily Misidentified as Escherichia coli
Sivertsen, Audun; Dyrhovden, Ruben; Tellevik, Marit Gjerde; Bruvold, Torbjørn Sæle; Nybakken, Eirik Jovall; Skutlaberg, Dag Harald; Skarstein, Ingerid; Kommedal, Øyvind (Journal article; Peer reviewed, 2022)We hereby present the first descriptions of human-invasive infections caused by Escherichia marmotae, a recently described species that encompasses the former “Escherichia cryptic clade V.” We describe four cases, one acute ... -
Genetic diversity of circulating rotavirus strains in Tanzania prior to the introduction of vaccination
Moyo, Sabrina John; Blomberg, Bjørn; Hanevik, Kurt; Kommedal, Øyvind; Vainio, Kirsti; Maselle, Samwel Y.; Langeland, Nina (Peer reviewed; Journal article, 2014-05-20)Background: Tanzania currently rolls out vaccination against rotavirus-diarrhea, a major cause of child illness and death. As the vaccine covers a limited number of rotavirus variants, this study describes the molecular ... -
High prevalence of faecal carriage of ESBL-producing enterobacteriaceae among children in Dar es Salaam, Tanzania
Tellevik, Marit Gjerde; Blomberg, Bjørn; Kommedal, Øyvind; Maselle, Samuel Y; Langeland, Nina; Moyo, Sabrina John (Peer reviewed; Journal article, 2016-12-09)Background: Faecal carriage of ESBL-producing bacteria is a potential risk for transmission and infection. Little is known about faecal carriage of antibiotic resistance in Tanzania. This study aimed to investigate the ... -
Horizontal plasmid transfer among klebsiella pneumoniae isolates is the key factor for dissemination of extended-spectrum β-lactamases among children in Tanzania
Pedersen, Torunn Annie; Tellevik, Marit Gjerde; Kommedal, Øyvind; Lindemann, Paul Christoffer; Moyo, Sabrina John; James Peter, Jessin Janice; Blomberg, Bjørn; Samuelsen, Ørjan; Langeland, Nina (Journal article; Peer reviewed, 2020)Increased knowledge about the role of horizontal gene transfer is key to improve our understanding of the spread of antimicrobial resistance (AMR) in human populations. We therefore studied the dissemination of the blaCTX-M-15 ... -
Impact of rapid molecular testing on diagnosis, treatment and management of community-acquired pneumonia in Norway: a pragmatic randomised controlled trial (CAPNOR)
Serigstad, Sondre; Ritz, Christian; Faurholt-Jepsen, Daniel; Markussen, Dagfinn Lunde; Ebbesen, Marit Helen; Kommedal, Øyvind; Bjørneklett, Rune Oskar; Heggelund, Lars; Clark, Tristan W.; van Werkhoven, Cornelis H.; Knoop, Siri Tandberg; Ulvestad, Elling; Grewal, Harleen (Journal article; Peer reviewed, 2022)Background: Community-acquired pneumonia (CAP) causes a large burden of disease. Due to difficulties in obtaining representative respiratory samples and insensitive standard microbiological methods, the microbiological ... -
Investigating the human jejunal microbiota
Villmones, Heidi Cecilie; Svanevik, Marius; Ulvestad, Elling; Stenstad, Tore; Anthonisen, Inger Lill; Nygaard, Randi Monsen; Dyrhovden, Ruben; Kommedal, Øyvind (Journal article; Peer reviewed, 2022)Descriptions of the small intestinal microbiota are deficient and conflicting. We aimed to get a reliable description of the jejunal bacterial microbiota by investigating samples from two separate jejunal segments collected ... -
Managing Contamination and Diverse Bacterial Loads in 16S rRNA Deep Sequencing of Clinical Samples: Implications of the Law of Small Numbers
Dyrhovden, Ruben; Rippin, Martin; Øvrebø, Kjell Kåre; Nygaard, Randi Monsen; Ulvestad, Elling; Kommedal, Øyvind (Journal article; Peer reviewed, 2021)In this article, we investigate patterns of microbial DNA contamination in targeted 16S rRNA amplicon sequencing (16S deep sequencing) and demonstrate how this can be used to filter background bacterial DNA in diagnostic ... -
Microbiological diagnosis of pleural infections: a comparative evaluation of a novel syndromic real-time PCR panel
Kommedal, Øyvind; Eagan, Tomas Mikal Lind; Fløtten, Øystein; Leegaard, Truls Michael; Siljan, William Ward; Fardal, Hilde; Bø, Bjørnar; Grøvan, Fredrik; Larssen, Kjersti Wik; Kildahl-Andersen, Arne; Hjetland, Reidar; Tilseth, Rune Hørgård; Hareide, Sølvi Kristine Øyen; Tellevik, Marit Gjerde; Dyrhovden, Ruben (Journal article; Peer reviewed, 2024)Current microbial diagnostics for pleural infections are insufficient. Studies using 16S targeted next-generation sequencing report that only 10%–16% of bacteria present are cultured and that 50%–78% of pleural fluids ... -
Pleural Empyema Caused by Streptococcus intermedius and Fusobacterium nucleatum: A Distinct Entity of Pleural Infections
Dyrhovden, Ruben; Eagan, Tomas Mikal Lind; Fløtten, Øystein; Siljan, William Ward; Leegaard, Truls Michael; Bø, Bjørnar; Fardal, Hilde; Grøvan, Fredrik; Kildahl-Andersen, Arne; Larssen, Kjersti Wik; Tilseth, Rune; Hjetland, Reidar; Løes, Sigbjørn Suk; Lindemark, Frode; Tellevik, Marit Gjerde; Breistein, Rebecca Irene; Kommedal, Øyvind (Journal article; Peer reviewed, 2023)Background Many community-acquired pleural infections are caused by facultative and anaerobic bacteria from the human oral microbiota. The epidemiology, clinical characteristics, pathogenesis, and etiology of such ... -
Prevalence and molecular characterisation of human adenovirus in diarrhoeic children in Tanzania; a case control study
Moyo, Sabrina John; Hanevik, Kurt; Blomberg, Bjørn; Kommedal, Øyvind; Nordbø, Svein Arne; Maselle, Samuel Y; Langeland, Nina (Peer reviewed; Journal article, 2014-12-12)Background: Human adenovirus (HAdV) causes acute diarrhoea sporadically, as well as in outbreaks. Understanding the prevalence and types of HAdV in diarrhoea is important for control and preventive measures, especially in ... -
Rapid syndromic PCR testing in patients with respiratory tract infections reduces time to results and improves microbial yield
Serigstad, Sondre; Grewal, Harleen; Ebbesen, Marit Helen; Kommedal, Øyvind; Heggelund, Lars; van Werkhoven, Werkhoven; Faurholt-Jepsen, Daniel; Clark, T.W.; Ritz, Christian; Ulvestad, Elling; Bjørneklett, Rune Oskar; Knoop, Siri Tandberg; Ravn, P.; Markussen, Dagfinn Lunde (Journal article; Peer reviewed, 2022)Lack of rapid and comprehensive microbiological diagnosis in patients with community acquired pneumonia (CAP) hampers appropriate antimicrobial therapy. This study evaluates the real-world performance of the BioFire FilmArray ... -
Repeated bronchoscopy in health and obstructive lung disease: is the airway microbiome stable?
Nielsen, Rune; Xue, Yaxin; Jonassen, Inge; Haaland, Ingvild; Kommedal, Øyvind; Wiker, Harald Gotten; Drengenes, Christine; Bakke, Per S.; Eagan, Tomas Mikal Lind (Journal article; Peer reviewed, 2021)Objective Little is known concerning the stability of the lower airway microbiome. We have compared the microbiota identified by repeated bronchoscopy in healthy subjects and patients with ostructive lung diseaseases ... -
Species Level Description of the Human Ileal Bacterial Microbiota
Villmones, Heidi Cecilie; Haug, Erik Skaaheim; Ulvestad, Elling; Grude, Nils; Stenstad, Tore; Halland, Adrian; Kommedal, Øyvind (Journal article; Peer reviewed, 2018)The small bowel is responsible for most of the body’s nutritional uptake and for the development of intestinal and systemic tolerance towards microbes. Nevertheless, the human small bowel microbiota has remained poorly ...