Blar i Bergen Open Research Archive på forfatter "Shanbhag, Siddharth"
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Bone regeneration in rat calvarial defects using dissociated or spheroid mesenchymal stromal cells in scaffold-hydrogel constructs
Shanbhag, Siddharth; Suliman, Salwa; Mohamed-Ahmed, Samih Salah Eldin Mahgoub; Kampleitner, Carina; Hassan, Mohamed Nageeb; Heimel, Patrick; Dobsak, Toni; Tangl, Stefan; Bolstad, Anne Isine; Mustafa, Kamal Babikeir Elnour (Journal article; Peer reviewed, 2021)Background Three-dimensional (3D) spheroid culture can promote the osteogenic differentiation of bone marrow mesenchymal stromal cells (BMSC). 3D printing offers the possibility to produce customized scaffolds for complex ... -
Brief communication: Effects of conditioned media from human platelet lysate cultured MSC on osteogenic cell differentiation in vitro
Shanbhag, Siddharth; Al-Sharabi, Niyaz Abdulbaqi Abdulmajid; Mohamed-Ahmed, Samih Salah Eldin Mahgoub; Gruber, Reinhard; Kristoffersen, Einar Klæboe; Mustafa, Kamal Babikeir Elnour (Journal article; Peer reviewed, 2022)Culturing mesenchymal stromal cells (MSC) in human platelet lysate (HPL) supplemented media can enhance their osteogenic differentiation potential. The objective of this study was to test the hypothesis that conditioned ... -
Ectopic Bone Tissue Engineering in Mice Using Human Gingiva or Bone Marrow-Derived Stromal/Progenitor Cells in Scaffold-Hydrogel Constructs
Shanbhag, Siddharth; Kampleitner, Carina; Mohamed-Ahmed, Samih Salah Eldin Mahgoub; Yassin, Mohammed Ahmed Alamin Yousif; Dongre, Harsh Nitin; Costea, Daniela Elena; Tangl, Stefan; Stavropoulos, Andreas; Bolstad, Anne Isine; Suliman, Salwa; Mustafa, Kamal Babikeir Elnour (Journal article; Peer reviewed, 2021-11-30)Three-dimensional (3D) spheroid culture can promote the osteogenic differentiation and bone regeneration capacity of mesenchymal stromal cells (MSC). Gingiva-derived progenitor cells (GPC) represent a less invasive alternative ... -
Functionalizing Collagen Membranes with MSC-Conditioned Media Promotes Guided Bone Regeneration in Rat Calvarial Defects
Shanbhag, Siddharth; Kampleitner, Carina; Al-Sharabi, Niyaz Abdulbaqi Abdulmajid; Mohamed-Ahmed, Samih Salah Eldin Mahgoub; Apaza Alccayhuaman, Karol Ali; Heimel, Patrick; Tangl, Stefan; Beinlich, Andreas Michael; Rana, Neha; Sanz, Mariano; Kristoffersen, Einar Klæboe; Mustafa, Kamal Babikeir Elnour; Gruber, Reinhard (Journal article; Peer reviewed, 2023-02-28)Functionalizing biomaterials with conditioned media (CM) from mesenchymal stromal cells (MSC) is a promising strategy for enhancing the outcomes of guided bone regeneration (GBR). This study aimed to evaluate the bone ... -
Influence of Bone Substitutes on Mesenchymal Stromal Cells in an Inflammatory Microenvironment
Shanbhag, Siddharth; Rana, Neha; Suliman, Salwa; Idris, Shaza Bushra Mohamed; Mustafa, Kamal Babikeir Elnour; Stavropoulos, Andreas (Journal article; Peer reviewed, 2022)Bone regeneration is driven by mesenchymal stromal cells (MSCs) via their interactions with immune cells, such as macrophages (MPs). Bone substitutes, e.g., bi-calcium phosphates (BCPs), are commonly used to treat bone ... -
Influence of platelet storage time on human platelet lysates and platelet lysate-expanded mesenchymal stromal cells for bone tissue engineering
Shanbhag, Siddharth; Mohamed Ahmed, Samih; Lunde, Turid Helen Felli; Suliman, Salwa; Bolstad, Anne Isine; Hervig, Tor; Mustafa, Kamal Babikeir Eln (Journal article; Peer reviewed, 2020)Background Human platelet lysate (HPL) is emerging as the preferred xeno-free supplement for the expansion of mesenchymal stromal cells (MSCs) for bone tissue engineering (BTE) applications. Due to a growing demand, the ... -
Patient experience following iliac crest alveolar bone gradfting av implant replacement
Gjerde, Cecilie Gudveig; Mustafa, Kamal Babikeir Eln; Gjengedal, Harald; Shanbhag, Siddharth; Neppelberg, Evelyn (Journal article; Peer reviewed, 2020-02-05)Background The objective of this study was to assess patient-reported outcomes such as satisfaction and quality of life after advanced alveolar bone augmentation with anterior iliac crest grafting and implant treatment ... -
Proteomic Analysis of Mesenchymal Stromal Cells Secretome in Comparison to Leukocyte- and Platelet-Rich Fibrin
Al-Sharabi, Niyaz Abdulbaqi Abdulmajid; Gruber, Reinhard; Sanz, Mariano; Mohamed-Ahmed, Samih Salah Eldin Mahgoub; Kristoffersen, Einar Klæboe; Mustafa, Kamal Babikeir Elnour; Shanbhag, Siddharth (Journal article; Peer reviewed, 2023)Secretomes of mesenchymal stromal cells (MSCs) are emerging as a novel growth factor (GF)-based strategy for periodontal and bone regeneration. The objective of this study was to compare the secretome of human bone marrow ... -
Scaffolds in Periodontal Regenerative Treatment
Yamada, Shuntaro; Shanbhag, Siddharth; Mustafa, Kamal Babikeir Elnour (Journal article, 2021) -
Spheroid Coculture of Human Gingiva-Derived Progenitor Cells With Endothelial Cells in Modified Platelet Lysate Hydrogels
Shanbhag, Siddharth; Elsebahy, Ahmad Rashad; Helgeland Nymark, Ellen; Suliman, Salwa; Davies, Catharina de Lange; Stavropoulos, Andreas; Bolstad, Anne Isine; Mustafa, Kamal Babikeir Elnour (Journal article; Peer reviewed, 2021-08-26)Cell coculture strategies can promote angiogenesis within tissue engineering constructs. This study aimed to test the angiogenic potential of human umbilical vein endothelial cells (HUVEC) cocultured with gingiva-derived ... -
The use of mesenchymal stromal cell secretome to enhance guided bone regeneration in comparison with leukocyte and platelet-rich fibrin
Shanbhag, Siddharth; Al-Sharabi, Niyaz Abdulbaqi Abdulmajid; Kampleitner, Carina; Mohamed-Ahmed, Samih Salah Eldin Mahgoub; Kristoffersen, Einar Klæboe; Tangl, Stefan; Mustafa, Kamal Babikeir Elnour; Gruber, Reinhard; Sanz, Mariano (Journal article; Peer reviewed, 2024)Objectives Secretomes of mesenchymal stromal cells (MSC) represent a novel strategy for growth-factor delivery for tissue regeneration. The objective of this study was to compare the efficacy of adjunctive use of ... -
Xeno-free 3D Culture of Mesenchymal Stromal Cells For Bone Tissue Engineering
Shanbhag, Siddharth (Doctoral thesis, 2020)Clinical translation of innovative regenerative approaches using mesenchymal stromal cells (MSCs) is urgently needed for the treatment of challenging bone defects. The overall aim of this thesis, comprising of one systematic ... -
Xeno-free spheroids of human gingiva-derived progenitor cells for bone tissue engineering
Shanbhag, Siddharth; Suliman, Salwa; Bolstad, Anne Isine; Stavropoulos, Andreas; Mustafa, Kamal Babikeir Eln (Journal article; Peer reviewed, 2020)Gingiva has been identified as a minimally invasive source of multipotent progenitor cells (GPCs) for use in bone tissue engineering (BTE). To facilitate clinical translation, it is important to characterize GPCs in xeno-free ...