Browsing Bergen Open Research Archive by Author "Steen, Ida Helene"
Now showing items 1-20 of 22
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Activation mechanism and activity of globupain, a thermostable C11 protease from the Arctic Mid-Ocean Ridge hydrothermal system
Røyseth, Victoria; M. Hurysz, Brianna; Kaczorowska, Anna-Karina; Dorawa, Sebastian; Fedøy, Anita-Elin; Arsin, Hasan; Sá M. Serafim, Mateus; A. Myers, Samuel; Werbowy, Olesia; Kaczorowski, Tadeusz; Stokke, Runar; O'Donoghue, Anthony J.; Steen, Ida Helene (Journal article; Peer reviewed, 2023)Deep-sea hydrothermal vents offer unique habitats for heat tolerant enzymes with potential new enzymatic properties. Here, we present the novel C11 protease globupain, which was prospected from a metagenome-assembled genome ... -
Adaptive evolution of viruses infecting marine microalgae (haptophytes), from acute infections to stable coexistence
Sandaa, Ruth-Anne; Saltvedt, Marius Rydningen; Dahle, Håkon; Wang, Haina; Våge, Selina; Blanc-Mathieu, Romain; Steen, Ida Helene; Grimsley, Nigel; Edvardsen, Bente; Ogata, Hiroyuki; Lawrence, Janice (Journal article; Peer reviewed, 2022)Collectively known as phytoplankton, photosynthetic microbes form the base of the marine food web, and account for up to half of the primary production on Earth. Haptophytes are key components of this phytoplankton community, ... -
An Arctic natural oil seep investigated from space to the seafloor
Panieri, Giuliana; Argentino, Claudio; Ramalho, Sofia P.; Vulcano, Francesca; Savini, Alessandra; Fallati, Luca; Brekke, Trond; Galimberti, Giulia; Riva, Federica; Balsa, João; Eilertsen, Mari Heggernes; Stokke, Runar; Steen, Ida Helene; Sahy, Diana; Kalenitchenko, Dimitri Stanislas Desire; Bünz, Stefan; Mattingsdal, Rune (Journal article; Peer reviewed, 2023)Due to climate change, decreasing ice cover and increasing industrial activities, Arctic marine ecosystems are expected to face higher levels of anthropogenic stress. To sustain healthy and productive ocean ecosystems, it ... -
Assessment of the carbon monoxide metabolism of the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus VC-16 by comparative transcriptome analyses
Hocking, William Peter; Roalkvam, Irene; Magnussen, Carina; Stokke, Runar; Steen, Ida Helene (Peer reviewed; Journal article, 2015)The hyperthermophilic, sulfate-reducing archaeon, Archaeoglobus fulgidus, utilizes CO as an energy source and it is resistant to the toxic effects of high CO concentrations. Herein, transcription profiles were obtained ... -
“Candidatus Ethanoperedens,” a thermophilic genus of archaea mediating the anaerobic oxidation of ethane
Hahn, Cedric Jasper; Laso-Pérez, Rafael; Vulcano, Francesca; Vaziourakis, Konstantinos-Marios; Stokke, Runar; Steen, Ida Helene; Teske, Andreas; Boetius, Antje; Liebeke, Manuel; Amann, Rudolf; Knittel, Katrin; Wegener, Gunter (Journal article; Peer reviewed, 2020)Cold seeps and hydrothermal vents deliver large amounts of methane and other gaseous alkanes into marine surface sediments. Consortia of archaea and partner bacteria thrive on the oxidation of these alkanes and its coupling ... -
Complete genome sequence of Lutibacter profoundi LP1T isolatet from an Arctic deep-sea hydrothermal vent system
Wissuwa, Juliane; Le Moine Bauer, Sven; Steen, Ida Helene; Stokke, Runar (Peer reviewed; Journal article, 2017-01-07)Lutibacter profundi LP1T within the family Flavobacteriaceae was isolated from a biofilm growing on the surface of a black smoker chimney at the Loki’s Castle vent field, located on the Arctic Mid-Ocean Ridge. The complete ... -
The complex structures of isocitrate dehydrogenase from Clostridium thermocellum and Desulfotalea psychrophila support a new active site locking mechanism
Leiros, Hanna-Kirsti S.; Fedøy, Anita-Elin; Leiros, Ingar; Steen, Ida Helene (Peer reviewed; Journal article, 2012)Isocitrate dehydrogenase (IDH) catalyzes the oxidative NAD(P)+-dependent decarboxylation of isocitrate into α-ketoglutarate and CO2 and is present in organisms spanning the biological range of temperature. We have solved ... -
Energy landscapes shape microbial communities in hydrothermal systems on the Arctic Mid-Ocean Ridge
Dahle, Håkon; Økland, Ingeborg Elisabet; Thorseth, Ingunn Hindenes; Pedersen, Rolf B.; Steen, Ida Helene (Peer reviewed; Journal article, 2015-01-09)Methods developed in geochemical modelling combined with recent advances in molecular microbial ecology provide new opportunities to explore how microbial communities are shaped by their chemical surroundings. Here, we ... -
Exploring codon adjustment strategies towards Escherichia coli-based production of viral proteins encoded by HTH1, a novel prophage of the marine bacterium Hypnocyclicus thermotrophus
Arsin, Hasan; Jasilionis, Andrius; Dahle, Håkon; Sandaa, Ruth-Anne; Stokke, Runar; Eva, Nordberg Karlsson; Steen, Ida Helene (Journal article; Peer reviewed, 2021)Marine viral sequence space is immense and presents a promising resource for the discovery of new enzymes interesting for research and biotechnology. However, bottlenecks in the functional annotation of viral genes and ... -
Finding a Needle in the Virus Metagenome Haystack - Micro-Metagenome Analysis Captures a Snapshot of the Diversity of a Bacteriophage Armoire
Ray, Jessica; Dondrup, Michael; Modha, Sejal; Steen, Ida Helene; Sandaa, Ruth-Anne; Clokie, Martha (Peer reviewed; Journal article, 2012-04-11)Viruses are ubiquitous in the oceans and critical components of marine microbial communities, regulating nutrient transfer to higher trophic levels or to the dissolved organic pool through lysis of host cells. Hydrothermal ... -
Hot Vents Beneath an Icy Ocean: The Aurora Vent Field, Gakkel Ridge, Revealed
Ramirez-Llodra, Eva; Argentino, Claudio; Baker, Maria; Boetius, Antje; Costa, Carolina; Dahle, Håkon; Denny, Emily Maria; Dessandier, Pierre-Antoine; Eilertsen, Mari Heggernes; Ferré, Benedicte; German, Cristopher R.; Hand, Kevin; Hilário, Ana; Hislop, Lawrence; Jamieson, John W.; Kalenitchenko, Dimitri; Mall, Achim; Panieri, Giuliana; Purser, Autun; Ramalho, Sofia P.; Reeves, Eoghan; Rolley, Leighton; Pereira, Samuel; de Azevedo Ribeiro, Pedro Miguel; Sert, Muhammed Fatih; Steen, Ida Helene; Stetzler, Marie Helene Paula; Stokke, Runar; Victorero, Lissette; Vulcano, Francesca; Vågenes, Stig; Waghorn, Kate Alyse; Bünz, Stefan (Journal article; Peer reviewed, 2022)Evidence of hydrothermal venting on the ultra-slow spreading Gakkel Ridge in the Central Arctic Ocean has been available since 2001, with first visual evidence of black smokers on the Aurora Vent Field obtained in 2014. ... -
Hydrothermal activity fuels microbial sulfate reduction in deep and distal marine settings along the Arctic Mid Ocean Ridges
Roerdink, Desiree Lisette; Vulcano, Francesca; Landro, Jan-Kristoffer; Moltubakk, Karen Elisabeth; Babel, Hannah Rose; Jørgensen, Steffen Leth; Baumberger, Tamara; Økland, Ingeborg Elisabet; Reeves, Eoghan; Thorseth, Ingunn Hindenes; Reigstad, Laila Johanne; Strauss, Harald; Steen, Ida Helene (Journal article; Peer reviewed, 2023)Microbial sulfate reduction is generally limited in the deep sea compared to shallower marine environments, but cold seeps and hydrothermal systems are considered an exception. Here, we report sulfate reduction rates and ... -
Identification and characterization of a hyperthermophilic GH9 cellulase from the Arctic Mid-Ocean Ridge vent field
Stepnov, Anton; Fredriksen, Lasse; Steen, Ida Helene; Stokke, Runar; Eijsink, Vincent (Peer reviewed; Journal article, 2019-09-06)A novel GH9 cellulase (AMOR_GH9A) was discovered by sequence-based mining of a unique metagenomic dataset collected at the Jan Mayen hydrothermal vent field. AMOR_GH9A comprises a signal peptide, a catalytic domain and a ... -
Identification of key components in the energy metabolism of Archaeoglobus fulgidus by transcriptome analyses
Hocking, William Peter; Stokke, Runar; Roalkvam, Irene; Steen, Ida Helene (Peer reviewed; Journal article, 2014-03-11)Energy conservation via the pathway of dissimilatory sulfate reduction is present in a diverse group of prokaryotes, but is most comprehensively studied in Deltaproteobacteria. In this study, whole-genome microarray analyses ... -
Isolation and complete genome sequence of the thermophilic Geobacillus sp. 12AMOR1 from an Arctic deep-sea hydrothermal vent site
Wissuwa, Juliane; Stokke, Runar; Fedøy, Anita-Elin; Lian, Kjersti; Smalås, Arne O.; Steen, Ida Helene (Peer reviewed; Journal article, 2016-02-24)Members of the genus Geobacillus have been isolated from a wide variety of habitats worldwide and are the subject for targeted enzyme utilization in various industrial applications. Here we report the isolation and complete ... -
Me, myself and I -Teachers’ self-motivation and sense of responsibility determine the use of active learning methods
Enberg, Katja; Steen, Ida Helene; Ellingsen, Ståle (Journal article; Peer reviewed, 2019)Extended use of laboratory and field courses makes biology a discipline considering itself as a habitual practitioner of active learning strategies. We investigated how widely the faculty at the Department of Biological ... -
Phylogenetic and functional diverse ANME-1 thrive in Arctic hydrothermal vents
Vulcano, Francesca; Hahn, Cedric Jasper; Roerdink, Desiree Lisette; Dahle, Håkon; Reeves, Eoghan; Wegener, Gunter; Steen, Ida Helene; Stokke, Runar (Journal article; Peer reviewed, 2022)The methane-rich areas, the Loki's Castle vent field and the Jan Mayen vent field at the Arctic Mid Ocean Ridge (AMOR), host abundant niches for anaerobic methane-oxidizers, which are predominantly filled by members of the ... -
Potential for homoacetogenesis via the Wood–Ljungdahl pathway in Korarchaeia lineages from marine hydrothermal vents
Vulcano, Francesca; Hribovšek, Petra; Denny, Emily Maria; Steen, Ida Helene; Stokke, Runar (Journal article; Peer reviewed, 2023)The Wood–Ljungdahl pathway (WLP) is a key metabolic component of acetogenic bacteria where it acts as an electron sink. In Archaea, despite traditionally being linked to methanogenesis, the pathway has been found in several ... -
Production, characterization, and application of an alginate lyase, AMOR_PL7A, from hot vents in the Arctic mid-ocean ridge
Vuoristo, Kiira; Fredriksen, Lasse; Oftebro, Maren; Arntzen, Magnus Øverlie; Aarstad, Olav Andreas; Stokke, Runar; Steen, Ida Helene; Hansen, Line Degn; Schüller, Reidar Barfod; Aachmann, Finn Lillelund; Horn, Svein Jarle; Eijsink, Vincent (Journal article; Peer reviewed, 2019)Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase ... -
Profundibacter amoris gen. nov., sp. nov., a new member of the Roseobacter clade isolated from Loki's Castle Vent Field on the Arctic Mid-Ocean Ridge
Le Moine Bauer, Sven; Sjøberg, Andreas Gilje; L´Haridon, Stephane; Stokke, Runar; Roalkvam, Irene; Steen, Ida Helene; Dahle, Håkon (Peer reviewed; Journal article, 2019)A bacterial strain, designated BAR1T, was isolated from a microbial mat growing on the surface of a barite chimney at the Loki’s Castle Vent Field, at a depth of 2216 m. Cells of strain BAR1T were rod-shaped, Gram-reacti ...