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Production, characterization, and application of an alginate lyase, AMOR_PL7A, from hot vents in the Arctic mid-ocean ridge

Vuoristo, Kiira; Fredriksen, Lasse; Oftebro, Maren; Arntzen, Magnus Øverlie; Aarstad, Olav Andreas; Stokke, Runar; Steen, Ida Helene; Hansen, Line Degn; Schüller, Reidar Barfod; Aachmann, Finn Lillelund; Horn, Svein Jarle; Eijsink, Vincent
Journal article, Peer reviewed
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URI
https://hdl.handle.net/11250/2738684
Date
2019
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  • Department of Biological Sciences [1843]
  • Registrations from Cristin [5667]
Original version
Journal of Agricultural and Food Chemistry. 2019, 67 (10), 2936–2945   10.1021/acs.jafc.8b07190
Abstract
Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase family 7 (PL7), which can be used to degrade brown seaweed, Saccharina latissima, at conditions also suitable for a commercial cellulase cocktail (Cellic CTec2). This enzyme, AMOR_PL7A, is a β-d-mannuronate specific (EC 4.2.2.3) endoacting alginate lyase, which degrades alginate and poly mannuronate within a broad range of pH, temperature and salinity. At 65 °C and pH 6.0, its Km and kcat values for sodium alginate are 0.51 ± 0.09 mg/mL and 7.8 ± 0.3 s–1 respectively. Degradation of seaweed with blends of Cellic CTec2 and AMOR_PL7A at 55 °C in seawater showed that the lyase efficiently reduces viscosity and increases glucose solublization. Thus, AMOR_PL7A may be useful in development of efficient protocols for enzymatic seaweed processing.
Publisher
ACS
Journal
Journal of Agricultural and Food Chemistry
Copyright
Copyright 2019 American Chemical Society

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