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dc.contributor.authorTolås, Anne Helene Paulsen
dc.date.accessioned2020-12-04T12:24:49Z
dc.date.issued2020-12-03
dc.date.submitted2020-12-02T23:00:08Z
dc.identifier.urihttps://hdl.handle.net/11250/2711958
dc.descriptionPostponed access: the file will be accessible after 2021-11-20
dc.description.abstractBacterial diseases in aquaculture lead to reduced health and mortality of fish as well as economic consequences for the producer. Antibiotics are an important antimicrobial to control bacterial diseases, however, concerns regarding the potential development and spread of antibiotic resistance genes are rising globally. Other solutions are considered, as the prophylactic or therapeutic use of bacteriophages, natural occurring viruses that infect and kill bacteria. This study aimed to isolate bacteriophages specific to pathogenic bacteria causing problems in Norwegian aquaculture. Among the bacteria causing disease in lumpfish (Cyclopterus lumpus L.) farming are Aeromonas salmonicida, Vibrio anguillarum and Pasteurella sp. Pasteurellosis is also an emerging disease in salmon (Salmo salar L.) farming. A collection of these bacteria were used to screen water samples for bacteriophages by using plaque assays. In addition to previously characterized isolates, bacteria from clinically sick salmon and lumpfish were isolated and sequenced by 16s rRNA sequencing. Water samples were collected from the environment and aquaculture sites. Pasteurella sp. was successfully isolated from clinically sick salmon suffering from pasteurellosis. From lumpfish diagnosed with vibriosis, isolation of V.anguillarum was expected, however, 16s rRNA sequencing showed the presence of Photobacterium spp. and Allivibrio logei. Isolation of bacteriophages was challenging, and target bacteriophages were not found in the majority of water samples. However, when samples from an aquaculture site with salmon diagnosed with pasteurellosis was tested, lysed bacteria were observed in plaque assays, indicating the presence of bacteriophages. Successful isolation of these bacteriophages were not achieved, and this posed a question if prophages present in the genome of Pasteurella sp. could have caused the plaques. Bacteriophages specific for V.anguillarum was not isolated, but a previously isolated bacteriophage showed an effect against pathogenic V.anguillarum previously isolated from lumpfish.
dc.language.isoeng
dc.publisherThe University of Bergen
dc.rightsCopyright the Author. All rights reserved
dc.subjectBacteriophages
dc.titleBacteriophages targeting pathogenic bacteria isolated from lumpfish (Cyclopterus lumpus L.) and Atlantic salmon (Salmo salar L.)
dc.typeMaster thesis
dc.date.updated2020-12-02T23:00:08Z
dc.rights.holderCopyright the Author. All rights reserved
dc.description.degreeMasteroppgave i fiskehelse
dc.description.localcodeFISK399
dc.description.localcodeMAMN-FISK
dc.subject.nus759906
fs.subjectcodeFISK399
fs.unitcode12-60-0
dc.date.embargoenddate2021-11-20


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