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dc.contributor.authorSandlund, Liveng
dc.contributor.authorNilsen, Frankeng
dc.contributor.authorMale, Runeeng
dc.contributor.authorGrotmol, Sindreeng
dc.contributor.authorKongshaug, Heidieng
dc.contributor.authorDalvin, Sussie Trineeng
dc.description.abstractThe salmon louse Lepeophtheirus salmonis (Copepoda, Caligidae) is an important parasite in the salmon farming industry in the Northern Hemisphere causing annual losses of hundreds of millions of dollars (US) worldwide. To facilitate development of a vaccine or other novel measures to gain control of the parasite, knowledge about molecular biological functions of L. salmonis is vital. In arthropods, a nuclear receptor complex consisting of the ecdysone receptor and the retinoid X receptor, ultraspiracle, are well known to be involved in a variety of both developmental and reproductive processes. To investigate the role of the ecdysone receptor in the salmon louse, we isolated and characterised cDNA with the 5′untranslated region of the predicted L. salmonis EcR (LsEcR). The LsEcR cDNA was 1608 bp encoding a 536 amino acid sequence that demonstrated high sequence similarities to other arthropod ecdysone receptors including Tribolium castaneum and Locusta migratoria. Moreover, in situ analysis of adult female lice revealed that the LsEcR transcript is localised in a wide variety of tissues such as ovaries, sub-cuticula and oocytes. Knock-down studies of LsEcR using RNA interference terminated egg production, indicating that the LsEcR plays important roles in reproduction and oocyte maturation. We believe this is the first report on the ecdysone receptor in the economically important parasite L. salmonis.en_US
dc.relation.ispartof<a href="" target="blank">Functional investigations of the Ecdysone Receptor and production of ecdysone in the salmon louse (Lepeophtheirus salmonis)</a>en_US
dc.rightsAttribution CC BY-NC-NDeng
dc.subjectSea liceeng
dc.subjectRNA interferenceeng
dc.subjectIn situeng
dc.titleMolecular characterisation of the salmon louse, Lepeophtheirus salmonis salmonis (Krøyer, 1837), ecdysone receptor with emphasis on functional studies of female reproductionen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2014 The Authorsen_US
dc.source.journalInternational Journal of Parasitology

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