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dc.contributor.authorPagarete, António
dc.contributor.authorGrébert, Théophile
dc.contributor.authorStepanova, Olga
dc.contributor.authorSandaa, Ruth-Anne
dc.contributor.authorBratbak, Gunnar
dc.PublishedViruses 2015, 7(7):3937-3953eng
dc.description.abstractNumbering in excess of 10 million per milliliter of water, it is now undisputed that aquatic viruses are one of the major factors shaping the ecology and evolution of Earth’s microbial world. Nonetheless, environmental viral diversity and roles remain poorly understood. Here we report the first thorough characterization of a virus (designated TsV) that infects the coastal marine microalga Tetraselmis striata. Unlike previously known microalgae-infecting viruses, TsV is a small (60 nm) DNA virus, with a 31 kb genome. From a range of eight different strains belonging to the Chlamydomonadaceae family, TsV was only able to infect T. striata. Gene expression dynamics revealed an up-regulation of viral transcripts already 1 h post-infection (p.i.). First clear signs of infection were observed 24 h p.i., with the appearance of viral factories inside the nucleus. TsV assembly was exclusively nuclear. TsV-N1 genome revealed very different from previously known algae viruses (Phycodnaviridae). Putative function and/or homology could be resolved for only 9 of the 33 ORFs encoded. Among those was a surprising DNA polymerase type Delta (only found in Eukaryotes), and two genes with closest homology to genes from human parasites of the urogenital tract. These results support the idea that the diversity of microalgae viruses goes far beyond the Phycodnaviridae family and leave the door open for future studies on implications of microalgae viruses for human health.en_US
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)en_US
dc.rightsAttribution CC BY 4.0eng
dc.subjectviral genomicseng
dc.subjectviral taxonomyeng
dc.subjectinfection dynamicseng
dc.subjectviral factoryeng
dc.subjecthorizontal gene transfereng
dc.titleTsv-N1: A novel DNA algal virus that infects tetraselmis striataen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2015 by the authors; licensee MDPI, Basel, Switzerlanden_US
dc.subject.nsiVDP::Matematikk og naturvitenskap: 400::Zoologiske og botaniske fag: 480::Parasittologi: 484
dc.subject.nsiVDP::Mathematics and natural scienses: 400::Zoology and botany: 480::Parasitology: 484
dc.subject.nsiVDP::Matematikk og Naturvitenskap: 400en_US

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Attribution CC BY 4.0
Except where otherwise noted, this item's license is described as Attribution CC BY 4.0