Show simple item record

dc.contributor.authorEgge, Elianne Sirnæs
dc.contributor.authorJohannessen, Torill Vik
dc.contributor.authorAndersen, Tom
dc.contributor.authorEikrem, Wenche
dc.contributor.authorBittner, Lucie
dc.contributor.authorLarsen, Aud
dc.contributor.authorSandaa, Ruth-Anne
dc.contributor.authorEdvardsen, Bente
dc.date.accessioned2016-03-04T09:00:01Z
dc.date.available2016-03-04T09:00:01Z
dc.date.issued2015-06
dc.PublishedMolecular Ecology 2015, 24(12):3026-3042eng
dc.identifier.issn1365-294Xen_US
dc.identifier.urihttp://hdl.handle.net/1956/11433
dc.description.abstractMicroalgae in the division Haptophyta play key roles in the marine ecosystem and in global biogeochemical processes. Despite their ecological importance, knowledge on seasonal dynamics, community composition and abundance at the species level is limited due to their small cell size and few morphological features visible under the light microscope. Here, we present unique data on haptophyte seasonal diversity and dynamics from two annual cycles, with the taxonomic resolution and sampling depth obtained with high-throughput sequencing. From outer Oslofjorden, S Norway, nano- and picoplanktonic samples were collected monthly for 2 years, and the haptophytes targeted by amplification of RNA/cDNA with Haptophyta-specific 18S rDNA V4 primers. We obtained 156 operational taxonomic units (OTUs), from c. 400.000 454 pyrosequencing reads, after rigorous bioinformatic filtering and clustering at 99.5%. Most OTUs represented uncultured and/or not yet 18S rDNA-sequenced species. Haptophyte OTU richness and community composition exhibited high temporal variation and significant yearly periodicity. Richness was highest in September–October (autumn) and lowest in April–May (spring). Some taxa were detected all year, such as Chrysochromulina simplex, Emiliania huxleyi and Phaeocystis cordata, whereas most calcifying coccolithophores only appeared from summer to early winter. We also revealed the seasonal dynamics of OTUs representing putative novel classes (clades HAP-3–5) or orders (clades D, E, F). Season, light and temperature accounted for 29% of the variation in OTU composition. Residual variation may be related to biotic factors, such as competition and viral infection. This study provides new, in-depth knowledge on seasonal diversity and dynamics of haptophytes in North Atlantic coastal waters.en_US
dc.language.isoengeng
dc.publisherWileyen_US
dc.rightsAttribution CC BY-NCeng
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/eng
dc.subjectDiversityeng
dc.subjectHaptophytaeng
dc.subjecthigh-throughputeng
dc.subjectSequencingeng
dc.subjectmultivariate analysiseng
dc.subjectPhytoplanktoneng
dc.subjectseasonalityeng
dc.titleSeasonal diversity and dynamics of haptophytes in the Skagerrak, Norway, explored by high-throughput sequencingen_US
dc.typePeer reviewed
dc.typeJournal article
dc.date.updated2015-12-30T17:08:11Z
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2015 The Authorsen_US
dc.identifier.doihttps://doi.org/10.1111/mec.13160
dc.identifier.cristin1263262
dc.subject.nsiVDP::Matematikk og naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497
dc.subject.nsiVDP::Mathematics and natural scienses: 400::Zoology and botany: 480::Marine biology: 497


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution CC BY-NC
Except where otherwise noted, this item's license is described as Attribution CC BY-NC