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dc.contributor.authorBrown, Matthew
dc.contributor.authorCamézuli, Stella
dc.contributor.authorDavenport, Russell J.
dc.contributor.authorPetelenz-Kurdziel, Elzbieta Anna
dc.contributor.authorØvreås, Lise
dc.contributor.authorCurtis, Tom
dc.PublishedWater Research 2015, 68:414-422eng
dc.description.abstractViruses may play a critical role in the microbial dynamics of activated sludge systems; however the difficulty of their quantification makes long term and large scale studies costly, timely and challenging. Thus a flow cytometric protocol was optimised and employed to determine virus abundance in activated sludge samples. The best flow cytometry signature and highest virus count was obtained by separating the indigenous floc-associated viruses using Tween 80 and sodium pyrophosphate, diluting the sample with Tris–EDTA and staining with SYBR Green II. Using the optimised protocol viral concentrations from 25 activated sludge plants were determined, with average concentrations of 2.35 × 109 mL−1 observed. Direct counts by transmission electron microscopy were highly correlated with flow cytometric counts (p = <0.05 and r2 = 0.77), with concentrations from both quantification methods comparable at the order of magnitude level. The high counting efficiency, ease of preparation and rapidity and reproducibility of analysis makes flow cytometric quantification of viruses in activated sludge ideal for routine investigation and thus invaluable in unravelling the complexity of phage host interactions in such systems.en_US
dc.publisherElsevier Ltd.en_US
dc.rightsAttribution CC BY 3.0eng
dc.subjectFlow cytometryeng
dc.subjectActivated sludgeeng
dc.subjectWastewater treatmenteng
dc.titleFlow cytometric quantification of viruses in activated sludgeen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2014 The Authorsen_US
dc.subject.nsiVDP::Matematikk og Naturvitenskap: 400en_US

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Attribution CC BY 3.0
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