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dc.contributor.authorOtterlei, Alexander
dc.contributor.authorBrevik, Øyvind Jakobsen
dc.contributor.authorJensen, Daniel
dc.contributor.authorDuesund, Henrik
dc.contributor.authorSommerset, Ingunn
dc.contributor.authorFrost, Petter
dc.contributor.authorMendoza, Julio
dc.contributor.authorMcKenzie, Peter
dc.contributor.authorNylund, Are
dc.contributor.authorApablaza, Patricia
dc.PublishedBMC Veterinary Research. 2016 Mar 15;12(1):55eng
dc.description.abstractBackground. The study presents the phenotypic and genetic characterization of selected P. salmonis isolates from Atlantic salmon and rainbow trout suffering from SRS (salmonid rickettsial septicemia) in Chile and in Canada. The phenotypic characterization of the P. salmonis isolates were based on growth on different agar media (including a newly developed medium), different growth temperatures, antibiotics susceptibility and biochemical tests. Results. This is the first study differentiating Chilean P. salmonis isolates into two separate genetic groups. Genotyping, based on 16S rRNA-ITS and concatenated housekeeping genes grouped the selected isolates into two clades, constituted by the Chilean strains, while the Canadian isolates form a branch in the phylogenetic tree. The latter consisted of two isolates that were different in both genetic and phenotypic characteristics. The phylogenies and the MLST do not reflect the origin of the isolates with respect to host species. The isolates included were heterogeneous in phenotypic tests. Conclusions. The genotyping methods developed in this study provided a tool for separation of P. salmonis isolates into distinct clades. The SRS outbreaks in Chile are caused by minimum two different genetic groups of P. salmonis. This heterogeneity should be considered in future development of vaccines against this bacterium in Chile. Two different strains of P. salmonis, in regards to genetic and phenotypic characteristics, can occur in the same contemporary outbreak of SRS.en_US
dc.publisherBioMed Centralen_US
dc.rightsAttribution CC BY 4.0eng
dc.subjectP. salmoniseng
dc.subject16S rDNA-ITSeng
dc.subjectHousekeeping geneseng
dc.titlePhenotypic and genetic characterization of Piscirickettsia salmonis from Chilean and Canadian salmonidsen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright Otterlei et al. 2016en_US
dc.subject.nsiVDP::Matematikk og Naturvitenskap: 400en_US

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Attribution CC BY 4.0
Except where otherwise noted, this item's license is described as Attribution CC BY 4.0