Vis enkel innførsel

dc.contributor.authorAl-Sharabi, Niyazen_US
dc.contributor.authorXue, Yingen_US
dc.contributor.authorUeda, Minoruen_US
dc.contributor.authorMustafa, Kamalen_US
dc.contributor.authorFristad, Ingeen_US
dc.PublishedDental Traumatology 2016, 32:231–239eng
dc.description.abstractAim. To investigate the effect of paracrine factors secreted from human bone marrow stromal cell (BMSC-CM) on pulpal and periodontal healing following immediate replantation of maxillary rat first molars. Material and Methods. Fifty maxillary rat first molars were elevated and replanted after 2 min. The left teeth were replanted without treatment, whereas BMSC-CM was injected into the right socket prior to replantation. Twelve un-operated teeth served as reference teeth. The expression of vascular endothelial growth factor A, alkaline phosphatase, Runt-related transcription factor 2 and osteoclast stimulating factor 1 was studied by real-time reverse transcription polymerase chain reaction at day 3 and 14. The dentin thickness together with Laminin- and PGP 9.5-immunoreactivity were studied after 3, 14 and 90 days. Results. Real-Time qRT-PCR data showed up-regulated expression of ALP mRNA in the socket specimens of conditioned medium treated replanted teeth after 3 days. No morphological differences were found for the expression of Laminin and PGP 9.5 between control and conditioned medium treated replanted teeth. At day 14, external cervical and surface root resorption was found in one BMSC-CM and one control tooth. At 90 days, all control replanted teeth had external cervical and surface root resorptions, whereas only one sample was seen among the conditioned medium treated teeth. At day 90, more extensive dentine formation with narrowing of the pulpal space was observed in the control compared with conditioned medium treated teeth. Conclusions. The present findings showed that BMSC-CM treatment reduced the number of replanted teeth with external root resorption and resulted in a significant reduction in new dentin formation.en_US
dc.publisherJohn Wiley & Sons Ltd.eng
dc.relation.ispartof<a href="" target="blank">Paracrine Effects of Mesenchymal Stem Cells on Dental Tissues - in vitro and in vivo studies</a>eng
dc.rightsAttribution CC BY-NC-ND 4.0eng
dc.subjectbone marrow stromal cell secreted factorseng
dc.subjecttooth replantationeng
dc.subjectexternal root resorptioneng
dc.subjectdentin thicknesseng
dc.titleInfluence of bone marrow stromal cell secreted molecules on pulpal and periodontal healing in replanted immature rat molarsen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2015 The Authors
dc.subject.nsiVDP::Medisinske Fag: 700en_US

Tilhørende fil(er)


Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel

Attribution CC BY-NC-ND 4.0
Med mindre annet er angitt, så er denne innførselen lisensiert som Attribution CC BY-NC-ND 4.0