dc.contributor.author | Sharma, Sunita | en_US |
dc.contributor.author | Sapkota, Dipak | en_US |
dc.contributor.author | Xue, Ying | en_US |
dc.contributor.author | Sun, Yang | en_US |
dc.contributor.author | Finne-Wistrand, Anna | en_US |
dc.contributor.author | Bruland, Ove | en_US |
dc.contributor.author | Mustafa, Kamal Babikeir Eln | en_US |
dc.date.accessioned | 2017-04-11T13:59:35Z | |
dc.date.available | 2017-04-11T13:59:35Z | |
dc.date.issued | 2016-01-25 | |
dc.Published | PLoS ONE 2016, 11(1):e0147507 | eng |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | https://hdl.handle.net/1956/15692 | |
dc.description.abstract | Selection of appropriate osteoinductive growth factors, suitable delivery method and proper supportive scaffold are critical for a successful outcome in bone tissue engineering using bone marrow stromal cells (BMSC). This study examined the molecular and functional effect of a combination of adenoviral mediated expression of bone morphogenetic protein-2 (BMP2) in BMSC and recently developed and characterized, biodegradable Poly(L-lactide-co-є-caprolactone){poly(LLA-co-CL)}scaffolds in osteogenic molecular changes and ectopic bone formation by using in vitro and in vivo approaches. Pathway-focused custom PCR array, validation using TaqMan based quantitative RT-PCR (qRT-PCR) and ALP staining showed significant up-regulation of several osteogenic and angiogenic molecules, including ALPL and RUNX2 in ad-BMP2 BMSC group grown in poly(LLA-co-CL) scaffolds both at 3 and 14 days. Micro CT and histological analyses of the subcutaneously implanted scaffolds in NOD/SCID mice revealed significantly increased radiopaque areas, percentage bone volume and formation of vital bone in ad-BMP2 scaffolds as compared to the control groups both at 2 and 8 weeks. The increased bone formation in the ad-BMP2 group in vivo was paralleled at the molecular level with concomitant over-expression of a number of osteogenic and angiogenic genes including ALPL, RUNX2, SPP1, ANGPT1. The increased bone formation in ad-BMP2 explants was not found to be associated with enhanced endochondral activity as evidenced by qRT-PCR (SOX9 and FGF2) and Safranin O staining. Taken together, combination of adenoviral mediated BMP-2 expression in BMSC grown in the newly developed poly(LLA-co-CL) scaffolds induced expression of osteogenic markers and enhanced bone formation in vivo. | en_US |
dc.language.iso | eng | eng |
dc.publisher | PLOS | eng |
dc.rights | Attribution CC BY | eng |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0 | eng |
dc.title | Adenoviral Mediated Expression of BMP2 by Bone Marrow Stromal Cells cultured in 3D Copolymer Scaffolds Enhances Bone Formation | en_US |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.date.updated | 2016-12-13T11:15:45Z | |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2016 The Author(s) | |
dc.identifier.doi | https://doi.org/10.1371/journal.pone.0147507 | |
dc.identifier.cristin | 1329770 | |