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dc.contributor.authorCollins, Andrew Richarden_US
dc.contributor.authorBalasubramanyam, Annangien_US
dc.contributor.authorRubio, Lauraen_US
dc.contributor.authorMarcos, Ricarden_US
dc.contributor.authorDorn, Marcoen_US
dc.contributor.authorMerker, Carolinen_US
dc.contributor.authorEstrela-Lopis, Irinaen_US
dc.contributor.authorCimpan, Mihaela Roxanaen_US
dc.contributor.authorIbrahim, Mohameden_US
dc.contributor.authorCimpan, Emilen_US
dc.contributor.authorOstermann, Melanieen_US
dc.contributor.authorSauter, Alexanderen_US
dc.contributor.authorEl Yamani, Naoualeen_US
dc.contributor.authorShaposhnikov, Sergeyen_US
dc.contributor.authorChevillard, Sylvieen_US
dc.contributor.authorPaget, Vincenten_US
dc.contributor.authorGrall, Romainen_US
dc.contributor.authorDelic, Jozoen_US
dc.contributor.authorGoñi- de-Cerio, Felipeen_US
dc.contributor.authorSuarez-Merino, Blancaen_US
dc.contributor.authorFessard, Valérieen_US
dc.contributor.authorHogeveen, Kevin Nen_US
dc.contributor.authorFjellsbø, Lise Mariaen_US
dc.contributor.authorRunden Pran, Eliseen_US
dc.contributor.authorBrzicova, Tanaen_US
dc.contributor.authorTopinka, Janen_US
dc.contributor.authorJoão Silva, Mariaen_US
dc.contributor.authorLeite, PEen_US
dc.contributor.authorRibeiro, ARen_US
dc.contributor.authorGranjeiro, JMen_US
dc.contributor.authorGrafström, Rolanden_US
dc.contributor.authorPrina-Mello, Adrieleen_US
dc.contributor.authorDusinska, Mariaen_US
dc.PublishedCollins AR, Balasubramanyam A, Rubio L, Marcos R, Dorn M, Merker C, Estrela-Lopis I, Cimpan MR, Ibrahim M, Cimpan E, Ostermann M, Sauter A, El Yamani N, Shaposhnikov S, Chevillard S, Paget V, Grall R, Delic J, Goñi- de-Cerio F, Suarez-Merino B, Fessard V, Hogeveen KN, Fjellsbø LM, Runden Pran E, Brzicova T, Topinka J, João Silva M, Leite P, Ribeiro A, Granjeiro J, Grafström R, Prina-Mello A, Dusinska M. High throughput toxicity screening and intracellular detection of nanomaterials. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology. 2017;9(1):e1413eng
dc.description.abstractWith the growing numbers of nanomaterials (NMs), there is a great demand for rapid and reliable ways of testing NM safety—preferably using in vitro approaches, to avoid the ethical dilemmas associated with animal research. Data are needed for developing intelligent testing strategies for risk assessment of NMs, based on grouping and read-across approaches. The adoption of high throughput screening (HTS) and high content analysis (HCA) for NM toxicity testing allows the testing of numerous materials at different concentrations and on different types of cells, reduces the effect of inter-experimental variation, and makes substantial savings in time and cost. HTS/HCA approaches facilitate the classification of key biological indicators of NM-cell interactions. Validation of in vitro HTS tests is required, taking account of relevance to in vivo results. HTS/HCA approaches are needed to assess dose- and time-dependent toxicity, allowing prediction of in vivo adverse effects. Several HTS/HCA methods are being validated and applied for NM testing in the FP7 project NANoREG, including Label-free cellular screening of NM uptake, HCA, High throughput flow cytometry, Impedance-based monitoring, Multiplex analysis of secreted products, and genotoxicity methods—namely High throughput comet assay, High throughput in vitro micronucleus assay, and γH2AX assay. There are several technical challenges with HTS/HCA for NM testing, as toxicity screening needs to be coupled with characterization of NMs in exposure medium prior to the test; possible interference of NMs with HTS/HCA techniques is another concern. Advantages and challenges of HTS/HCA approaches in NM safety are discussed.en_US
dc.rightsAttribution CC BY-NC-NDeng
dc.titleHigh throughput toxicity screening and intracellular detection of nanomaterialsen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2016 The Author(s)
dc.source.journalWiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology
dc.relation.projectEU: 310584
dc.relation.projectNorges forskningsråd: 239199/O70
dc.relation.projectNorges forskningsråd: 246672

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