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dc.contributor.authorTrösse, Christiane
dc.contributor.authorNilsen, Frank
dc.contributor.authorDalvin, Sussie Trine
dc.PublishedTrösse C, Nilsen F, Dalvin ST. RNA interference mediated knockdown of the KDEL receptor and COPB2 inhibits digestion and reproduction in the parasitic copepod Lepeophtheirus salmonis. Comparative Biochemistry and Physiology - Part B: Biochemistry & Molecular Biology. 2014;170:1-9eng
dc.description.abstractRetrograde transport of proteins from the endoplasmic reticulum to the Golgi is an essential part of the secretory pathway that all newly synthesised secreted and membrane proteins in eukaryotic cells undergo. The aim of this study was to characterise two components of the retrograde transport pathway in the parasitic copepod Lepeophtheirus salmonis (salmon louse) on a molecular and functional level. LsKDELR and LsCOPB2 were confirmed to be the salmon louse homologues of the chosen target proteins by sequence similarity. Ontogenetic analysis by qRT-PCR revealed the highest expression levels of both genes in adult females and the earliest larval stage. LsKDELR and LsCOPB2 localisation in adult females was detected by immunofluorescence and in situ hybridisation, respectively. Both LsKDELR and LsCOPB2 were found in the ovaries, the oocytes and the gut. LsKDELR and LsCOPB2 were knocked down by RNA interference in preadult females, which was confirmed by qRT-PCR. LsCOPB2 knockdown lice had a significantly higher mortality and failed to develop normally, while both LsCOPB2 and LsKDELR knockdown caused disturbed digestion and the absence of egg strings. This shows the potential of LsKDELR and LsCOPB2 as suitable target candidates for new pest control methods.en_US
dc.rightsAttribution CC BY-NC-NDeng
dc.subjectSea liceeng
dc.subjectCOPI beta primeeng
dc.subjectRetrograde transporteng
dc.titleRNA interference mediated knockdown of the KDEL receptor and COPB2 inhibits digestion and reproduction in the parasitic copepod Lepeophtheirus salmonisen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2014 Elsevieren_US
dc.source.journalComparative Biochemistry and Physiology - Part B: Biochemistry & Molecular Biology
dc.relation.projectNorges forskningsråd: 203513
dc.relation.projectNorges forskningsråd: 199778
dc.relation.projectNorges forskningsråd: 203513/O30
dc.subject.nsiVDP::Matematikk og naturvitenskap: 400::Zoologiske og botaniske fag: 480::Parasittologi: 484
dc.subject.nsiVDP::Mathematics and natural scienses: 400::Zoology and botany: 480::Parasitology: 484

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