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dc.contributor.authorJacobsen, Carsten Suhr
dc.contributor.authorNielsen, Tue Kjærgaard
dc.contributor.authorVester, Jan Kjølhede
dc.contributor.authorStougaard, Peter
dc.contributor.authorNielsen, Jeppe Lund
dc.contributor.authorVoriskova, Jana
dc.contributor.authorWinding, Anne
dc.contributor.authorBaldrian, Petr
dc.contributor.authorLiu, Binbin
dc.contributor.authorFrostegård, Åsa
dc.contributor.authorPedersen, Dorthe
dc.contributor.authorTveit, Alexander Tøsdal
dc.contributor.authorSvenning, Mette Marianne
dc.contributor.authorTebbe, Christoph C.
dc.contributor.authorØvreås, Lise
dc.contributor.authorJakobsen, Pia Bach
dc.contributor.authorBlazewicz, Steven J.
dc.contributor.authorHubablek, Valerie
dc.contributor.authorBertilsson, Stefan
dc.contributor.authorHansen, Lars Hestbjerg
dc.contributor.authorCary, S. Craig
dc.contributor.authorHolben, William E.
dc.contributor.authorEkelund, Flemming
dc.contributor.authorBælum, Jacob
dc.PublishedJacobsen, Nielsen, Vester, Stougaard P, Nielsen JL, Voriskova, Winding A, Baldrian P, Liu B, Frostegård ÅF, Pedersen D, Tveit AT, Svenning MM, Tebbe, Øvreås L, Jakobsen, Blazewicz, Hubablek, Bertilsson S, Hansen, Cary SC, Holben, Ekelund F, Bælum J. Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples. Scientific Reports. 2018;8:5711:1-6eng
dc.description.abstractHere we show that a commercial blocking reagent (G2) based on modified eukaryotic DNA significantly improved DNA extraction efficiency. We subjected G2 to an inter-laboratory testing, where DNA was extracted from the same clay subsoil using the same batch of kits. The inter-laboratory extraction campaign revealed large variation among the participating laboratories, but the reagent increased the number of PCR-amplified16S rRNA genes recovered from biomass naturally present in the soils by one log unit. An extensive sequencing approach demonstrated that the blocking reagent was free of contaminating DNA, and may therefore also be used in metagenomics studies that require direct sequencing.en_US
dc.publisherSpringer Natureen_US
dc.rightsAttribution CC BYeng
dc.subjectEcological geneticseng
dc.subjectSoil microbiologyeng
dc.titleInter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samplesen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright The Author(s) 2018en_US
dc.source.journalScientific Reports

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