dc.contributor.author | Arnesen, Thomas | eng |
dc.contributor.author | Betts, Matthew J. | eng |
dc.contributor.author | Pendino, Frédéric | eng |
dc.contributor.author | Liberles, David A. | eng |
dc.contributor.author | Anderson, Dave | eng |
dc.contributor.author | Caro, Jaime | eng |
dc.contributor.author | Kong, Xianguo | eng |
dc.contributor.author | Varhaug, Jan Erik | eng |
dc.contributor.author | Lillehaug, Johan | eng |
dc.date.accessioned | 2006-10-25T11:26:26Z | |
dc.date.available | 2006-10-25T11:26:26Z | |
dc.date.issued | 2006-04-25 | eng |
dc.identifier.issn | 1471-2091 | en_US |
dc.identifier.uri | https://hdl.handle.net/1956/1911 | |
dc.description.abstract | Background: Protein acetylation is increasingly recognized as an important mechanism regulating a variety of cellular functions. Several human protein acetyltransferases have been characterized, most of them catalyzing ε-acetylation of histones and transcription factors. We recently described the human protein acetyltransferase hARD1 (human Arrest Defective 1). hARD1 interacts with NATH (N-Acetyl Transferase Human) forming a complex expressing protein N-terminal α-acetylation activity. Results: We here describe a human protein, hARD2, with 81 % sequence identity to hARD1. The gene encoding hARD2 most likely originates from a eutherian mammal specific retrotransposition event. hARD2 mRNA and protein are expressed in several human cell lines. Immunoprecipitation experiments show that hARD2 protein potentially interacts with NATH, suggesting that hARD2-NATH complexes may be responsible for protein N-α-acetylation in human cells. In NB4 cells undergoing retinoic acid mediated differentiation, the level of endogenous hARD1 and NATH protein decreases while the level of hARD2 protein is stable. Conclusion: A human protein N-α-acetyltransferase is herein described. ARD2 potentially complements the functions of ARD1, adding more flexibility and complexity to protein N-α-acetylation in human cells as compared to lower organisms which only have one ARD. | en_US |
dc.format.extent | 1064704 bytes | eng |
dc.format.mimetype | application/pdf | eng |
dc.language.iso | eng | eng |
dc.publisher | BioMed Central | en_US |
dc.rights | Attribution CC BY | eng |
dc.rights.uri | http://creativecommons.org/licenses/by/2.0 | eng |
dc.title | Characterization of hARD2, a processed hARD1 gene duplicate, encoding a human protein N-α-acetyltransferase | en_US |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2006 Arnesen et al; licensee BioMed Central Ltd. | en_US |
dc.source.articlenumber | 13 | |
dc.identifier.doi | https://doi.org/10.1186/1471-2091-7-13 | |
dc.identifier.cristin | 375218 | |
dc.source.journal | BMC Biochemistry | |
dc.source.40 | 7 | |
dc.subject.nsi | VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Molekylærbiologi: 473 | nob |