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dc.contributor.authorNuñez-Ortiz, Noelia
dc.contributor.authorMoore, Lindsey
dc.contributor.authorJarungsriapisit, Jiraporn
dc.contributor.authorNilsen, Tom Ole
dc.contributor.authorStefansson, Sigurd Olav
dc.contributor.authorMorton, Hugh Craig
dc.contributor.authorTaranger, Geir Lasse
dc.contributor.authorSecombes, Christopher J.
dc.contributor.authorPatel, Sonal
dc.date.accessioned2019-04-26T11:38:18Z
dc.date.available2019-04-26T11:38:18Z
dc.date.issued2018-11
dc.PublishedNuñez-Ortiz, Moore L, Jarungsriapisit J, Nilsen TO, Stefansson SO, Morton HC, Taranger GL, Secombes CJ, Patel S. Atlantic salmon post-smolts adapted for a longer time to seawater develop an effective humoral and cellular immune response against Salmonid alphavirus. Fish and Shellfish Immunology. 2018;82:579-590eng
dc.identifier.issn1050-4648en_US
dc.identifier.issn1095-9947en_US
dc.identifier.urihttp://hdl.handle.net/1956/19414
dc.descriptionUnder embargo until: 31.08.2019
dc.description.abstractSalmonid alphavirus (SAV) causes pancreas disease (PD) in Atlantic salmon (Salmo salar L.) and disease outbreaks are mainly detected after seawater transfer. The influence of the smoltification process on the immune responses, specifically the adaptive response of Atlantic salmon after SAV infection, is not fully understood. In this study, Atlantic salmon post-smolts were infected by either bath immersion (BI) or intramuscular injection (IM) with SAV subtype 3, 2 weeks (Phase A) or 9 weeks (Phase B) after seawater transfer. The transcript levels of genes related to cellular, humoral and inflammatory responses were evaluated on head kidney samples collected at 3, 7, 14, 21, and 28 days post-infection (dpi). Corresponding negative control groups (CT) were established accordingly. Significant differences were found between both phases and between the IM and BI groups. The anti-inflammatory cytokine IL-10 was up-regulated in Phase A at a higher level than in Phase B. High mRNA levels of the genes RIG-1, SOCS1 and STAT1 were observed in all groups except the BI-B group (BI-Phase B). Moreover, the IM-B group showed a higher regulation of genes related to cellular responses, such as CD40, MHCII, and IL-15, that indicated the activation of a strong cell-mediated immune response. CD40 mRNA levels were elevated one week earlier in the BI-B group than in the BI-A group (BI-Phase A). A significant up-regulation of IgM and IgT genes was seen in both IM groups, but the presence of neutralizing antibodies to SAV was detected only in Phase B fish at 21 and 28 dpi. In addition, we found differences in the basal levels of some of the analysed genes between non-infected control groups of both phases. Findings suggest that Atlantic salmon post-smolts adapted for a longer time to seawater before they come into contact with SAV, developed a stronger humoral and cell-mediated immune response during a SAV infection.en_US
dc.language.isoengeng
dc.publisherElsevieren_US
dc.rightsAttribution CC BY-NC-NDeng
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/eng
dc.subjectSalmo Salareng
dc.subjectBath immersioneng
dc.subjectAdaptive responseeng
dc.subjectSmoltificationeng
dc.subjectPancreas diseaseeng
dc.subjectImmunoglobulineng
dc.subjectB cellseng
dc.subjectRIG-1eng
dc.subjectSOCS1eng
dc.subjectCD40eng
dc.subjectInterleukineng
dc.titleAtlantic salmon post-smolts adapted for a longer time to seawater develop an effective humoral and cellular immune response against Salmonid alphavirusen_US
dc.typePeer reviewed
dc.typeJournal article
dc.date.updated2018-11-08T15:15:22Z
dc.description.versionacceptedVersionen_US
dc.rights.holderCopyright 2018 Elsevieren_US
dc.identifier.doihttps://doi.org/10.1016/j.fsi.2018.08.059
dc.identifier.cristin1612518
dc.source.journalFish and Shellfish Immunology
dc.relation.projectNorges forskningsråd: 224885


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