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dc.contributor.authorWeigand, Hannah
dc.contributor.authorBeermann, Arne J.
dc.contributor.authorČiampor, Fedor
dc.contributor.authorCosta, Filipe O.
dc.contributor.authorCsabai, Zoltán
dc.contributor.authorDuarte, Sofia
dc.contributor.authorGeiger, Matthias F.
dc.contributor.authorGrabowski, Michał
dc.contributor.authorRimet, Frédéric
dc.contributor.authorRulik, Björn
dc.contributor.authorStrand, Malin
dc.contributor.authorSzucsich, Nicholaus
dc.contributor.authorWeigand, Alexander M.
dc.contributor.authorWillassen, Endre
dc.contributor.authorWyler, Sofia A.
dc.contributor.authorBouchez, Agnès
dc.contributor.authorBorja, Ángel
dc.contributor.authorČiamporová-Zaťovičová, Zuzana
dc.contributor.authorFerreira, Sónia
dc.contributor.authorDijkstra, Klaas-Douwe B.
dc.contributor.authorEisendle, Ursula
dc.contributor.authorFreyhof, Jörg
dc.contributor.authorGadawski, Piotr
dc.contributor.authorGraf, Wolfram
dc.contributor.authorHaegerbaeumer, Arne
dc.contributor.authorvan der Hoorn, Berry B.
dc.contributor.authorJaposhvili, Bella
dc.contributor.authorKeresztes, Lujza
dc.contributor.authorKeskin, Emre
dc.contributor.authorLeese, Florian
dc.contributor.authorMacher, Jan N.
dc.contributor.authorMamos, Tomasz
dc.contributor.authorPaz, Guy
dc.contributor.authorPešić, Vladimir
dc.contributor.authorPfannkuchen, Daniela Maric
dc.contributor.authorPfannkuchen, Martin Andreas
dc.contributor.authorPrice, Benjamin W.
dc.contributor.authorRinkevich, Buki
dc.contributor.authorTeixeira, Marcos A.L.
dc.contributor.authorVárbíró, Gábor
dc.contributor.authorEkrem, Torbjørn
dc.PublishedWeigand, Beermann, Čiampor F, Costa FO, Csabai, Duarte S, Geiger MF, Grabowski, Rimet, Rulik B, Strand M, Szucsich, Weigand, Willassen E, Wyler, Bouchez, Borja Á, Čiamporová-Zaťovičová, Ferreira S, Dijkstra KB, Eisendle, Freyhof J, Gadawski, Graf W, Haegerbaeumer, van der Hoorn, Japoshvili, Keresztes, Keskin E, Leese F, Macher, Mamos, Paz, Pešić, Pfannkuchen, Pfannkuchen, Price, Rinkevich, Teixeira, Várbíró, Ekrem T. DNA barcode reference libraries for the monitoring of aquatic biota in Europe: Gap-analysis and recommendations for future work. Science of the Total Environment. 2019;678:499-524.eng
dc.description.abstractEffective identification of species using short DNA fragments (DNA barcoding and DNA metabarcoding) requires reliable sequence reference libraries of known taxa. Both taxonomically comprehensive coverage and content quality are important for sufficient accuracy. For aquatic ecosystems in Europe, reliable barcode reference libraries are particularly important if molecular identification tools are to be implemented in biomonitoring and reports in the context of the EU Water Framework Directive (WFD) and the Marine Strategy Framework Directive (MSFD). We analysed gaps in the two most important reference databases, Barcode of Life Data Systems (BOLD) and NCBI GenBank, with a focus on the taxa most frequently used in WFD and MSFD. Our analyses show that coverage varies strongly among taxonomic groups, and among geographic regions. In general, groups that were actively targeted in barcode projects (e.g. fish, true bugs, caddisflies and vascular plants) are well represented in the barcode libraries, while others have fewer records (e.g. marine molluscs, ascidians, and freshwater diatoms). We also found that species monitored in several countries often are represented by barcodes in reference libraries, while species monitored in a single country frequently lack sequence records. A large proportion of species (up to 50%) in several taxonomic groups are only represented by private data in BOLD. Our results have implications for the future strategy to fill existing gaps in barcode libraries, especially if DNA metabarcoding is to be used in the monitoring of European aquatic biota under the WFD and MSFD. For example, missing species relevant to monitoring in multiple countries should be prioritized for future collaborative programs. We also discuss why a strategy for quality control and quality assurance of barcode reference libraries is needed and recommend future steps to ensure full utilisation of metabarcoding in aquatic biomonitoring.en_US
dc.rightsAttribution CC BY 4.0eng
dc.subjectDNA barcodingeng
dc.subjectReference libraryeng
dc.subjectBiological monitoringeng
dc.subjectQuality assuranceeng
dc.titleDNA barcode reference libraries for the monitoring of aquatic biota in Europe : Gap-analysis and recommendations for future workeng
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.rights.holderCopyright 2019 The Author(s)eng
dc.source.journalScience of the Total Environment
dc.relation.projectNorges forskningsråd: 226134
dc.relation.projectMiljødirektoratet: 15040013
dc.relation.projectNorges forskningsråd: 243791
dc.relation.projectNorges forskningsråd: 243791/E50

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Attribution CC BY 4.0
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