Trans-splicing of mRNAs links gene transcription to translational control regulated by mTOR
Journal article, Peer reviewed
MetadataShow full item record
Original versionDanks GB, Galbiati H, Raasholm M, Torres Cleuren YN, Valen E, Navratilova P, Thompson E. Trans-splicing of mRNAs links gene transcription to translational control regulated by mTOR. BMC Genomics. 2019;20:908 https://doi.org/10.1186/s12864-019-6277-x
Background: In phylogenetically diverse organisms, the 5′ ends of a subset of mRNAs are trans-spliced with a spliced leader (SL) RNA. The functions of SL trans-splicing, however, remain largely enigmatic. Results: We quantified translation genome-wide in the marine chordate, Oikopleura dioica, under inhibition of mTOR, a central growth regulator. Translation of trans-spliced TOP mRNAs was suppressed, consistent with a role of the SL sequence in nutrient-dependent translational control of growth-related mRNAs. Under crowded, nutrient-limiting conditions, O. dioica continued to filter-feed, but arrested growth until favorable conditions returned. Upon release from unfavorable conditions, initial recovery was independent of nutrient-responsive, trans-spliced genes, suggesting animal density sensing as a first trigger for resumption of development. Conclusion: Our results are consistent with a proposed role of trans-splicing in the coordinated translational down-regulation of nutrient-responsive genes under growth-limiting conditions.