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dc.contributor.authorHorn, Ivo R.
dc.contributor.authorKenens, Y.
dc.contributor.authorPalmblad, Magnus
dc.contributor.authorvan der Plas-Duivesteijn, Suzanne J
dc.contributor.authorLangeveld, Bram W.
dc.contributor.authorDalebout, Hans
dc.contributor.authorMeijer, Hanneke
dc.contributor.authorMarissen, Rob J.
dc.contributor.authorFischer, Anja
dc.contributor.authorFlorens, F.B. Vincent
dc.contributor.authorNiemann, Jonas
dc.contributor.authorRijsdijk, Kenneth F.
dc.contributor.authorSchulp, Anne S.
dc.contributor.authorLaros, Jeroen F.J.
dc.contributor.authorGravendeel, Barbara
dc.PublishedHorn, Kenens, Palmblad M, van der Plas-Duivesteijn, Langeveld, Dalebout, Meijer H.J.M., Marissen, Fischer, Florens FV, Niemann J, Rijsdijk KF, Schulp AS, Laros, Gravendeel B. Palaeoproteomics of fossil bird bones for taxonomic classification. Zoological Journal of the Linnean Society. 2019;186(3):650-665eng
dc.description.abstractWe used proteomic profiling to taxonomically classify extinct, alongside extant bird species using mass spectrometry on ancient bone-derived collagen chains COL1A1 and COL1A2. Proteins of Holocene and Late Pleistocene-aged bones from dodo (Raphus cucullatus) and great auk (Pinguinus impennis), as well as bones from chicken (Gallus gallus), rock dove (Columba livia), zebra finch (Taeniopygia guttata) and peregrine falcon (Falco peregrinus), of various ages ranging from the present to 1455 years old were analysed. HCl and guandine-HCL-based protein extractions from fresh bone materials yielded up to 60% coverage of collagens COL1A1 and COL1A2, and extractions from ancient materials yielded up to 46% coverage of collagens COL1A1 and COL1A2. Data were retrieved from multiple peptide sequences obtained from different specimens and multiple extractions. Upon alignment, and in line with the latest evolutionary insights, protein data obtained from great auk grouped with data from a recently sequenced razorbill (Alca torda) genome. Similarly, protein data obtained from bones of dodo and modern rock dove grouped in a single clade. Lastly, protein data obtained from chicken bones, both from ancient and fresh materials, grouped as a separate, basal clade. Our proteomic analyses enabled taxonomic classification of all ancient bones, thereby complementing phylogenetics based on DNA.en_US
dc.publisherOxford University Presseng
dc.rightsAttribution CC BY-NCeng
dc.titlePalaeoproteomics of fossil bird bones for taxonomic classificationeng
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.rights.holderCopyright Zoological Journal of the Linnean Society 2019eng
dc.source.journalZoological Journal of the Linnean Society

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