Enzyme histochemical studies of acid phosphatise isoenzymes in induced heterotopic bone formation in guinea pigs

Abstract

A bone induction model was used to study the effect of different pretreatments and inhibitors added to the substrate of acid phosphatise. Two different acid phosphatises (AcP) were demonstrated. The AcP of matrix-producing cells exhibited resistance to pre-treatment with EDTA while it was sensitive to tartrate added to the incubation medium. The AcP in dentin resorbing cells was EDTA sensitive but resistant to tartrate. Both resorbing and matrix-producing cells were generally sensitive to fluoride, copper and molybdate. In some of the dentionoclasts, fluoride resistant AcP was observed. The results support previous investigations on normal bone formation suggesting that two different isoenzymes of AcP exists. One of these seems to be related to matrix production and mineralization of the new heterotopic