Vis enkel innførsel

dc.contributor.authorRudolf, Jerneja
dc.contributor.authorDondorp, Daniel
dc.contributor.authorCanon, Louise
dc.contributor.authorTieo, Sonia
dc.contributor.authorChatzigeorgiou, Marios
dc.date.accessioned2020-04-24T12:11:21Z
dc.date.available2020-04-24T12:11:21Z
dc.date.issued2019-02-20
dc.PublishedRudolf J, Dondorp D, Canon L, Tieo S, Chatzigeorgiou M. Automated behavioural analysis reveals the basic behavioural repertoire of the urochordate Ciona intestinalis. Scientific Reports. 2019;9(2416):2416eng
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/1956/21992
dc.description.abstractQuantitative analysis of animal behaviour in model organisms is becoming an increasingly essential approach for tackling the great challenge of understanding how activity in the brain gives rise to behaviour. Here we used automated image-based tracking to extract behavioural features from an organism of great importance in understanding the evolution of chordates, the free-swimming larval form of the tunicate Ciona intestinalis, which has a compact and fully mapped nervous system composed of only 231 neurons. We analysed hundreds of videos of larvae and we extracted basic geometric and physical descriptors of larval behaviour. Importantly, we used machine learning methods to create an objective ontology of behaviours for C. intestinalis larvae. We identified eleven behavioural modes using agglomerative clustering. Using our pipeline for quantitative behavioural analysis, we demonstrate that C. intestinalis larvae exhibit sensory arousal and thigmotaxis. Notably, the anxiotropic drug modafinil modulates thigmotactic behaviour. Furthermore, we tested the robustness of the larval behavioural repertoire by comparing different rearing conditions, ages and group sizes. This study shows that C. intestinalis larval behaviour can be broken down to a set of stereotyped behaviours that are used to different extents in a context-dependent manner.en_US
dc.language.isoengeng
dc.publisherSpringer Natureeng
dc.rightsAttribution CC BYeng
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/eng
dc.titleAutomated behavioural analysis reveals the basic behavioural repertoire of the urochordate Ciona intestinaliseng
dc.typeJournal articleeng
dc.typePeer reviewedeng
dc.date.updated2020-01-10T11:11:20Z
dc.description.versionpublishedVersion
dc.rights.holderCopyright © 2019, Springer Natureeng
dc.identifier.doihttps://doi.org/10.1038/s41598-019-38791-5
dc.identifier.cristin1681964
dc.source.journalScientific Reports


Tilhørende fil(er)

Thumbnail

Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel

Attribution CC BY
Med mindre annet er angitt, så er denne innførselen lisensiert som Attribution CC BY