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dc.contributor.authorStepnov, Anton
dc.contributor.authorFredriksen, Lasse
dc.contributor.authorSteen, Ida Helene
dc.contributor.authorStokke, Runar
dc.contributor.authorEijsink, Vincent
dc.PublishedStepnov, Fredriksen, Steen, Stokke, Eijsink. Identification and characterization of a hyperthermophilic GH9 cellulase from the Arctic Mid-Ocean Ridge vent field. PLOS ONE. 2019;14(9):e0222216eng
dc.description.abstractA novel GH9 cellulase (AMOR_GH9A) was discovered by sequence-based mining of a unique metagenomic dataset collected at the Jan Mayen hydrothermal vent field. AMOR_GH9A comprises a signal peptide, a catalytic domain and a CBM3 cellulose-binding module. AMOR_GH9A is an exceptionally stable enzyme with a temperature optimum around 100°C and an apparent melting temperature of 105°C. The novel cellulase retains 64% of its activity after 4 hours of incubation at 95°C. The closest characterized homolog of AMOR_GH9A is TfCel9A, a processive endocellulase from the model thermophilic bacterium Thermobifida fusca (64.2% sequence identity). Direct comparison of AMOR_GH9A and TfCel9A revealed that AMOR_GH9A possesses higher activity on soluble and amorphous substrates (phosphoric acid swollen cellulose, konjac glucomannan) and has an ability to hydrolyse xylan that is lacking in TfCel9A.en_US
dc.rightsAttribution CC BYeng
dc.titleIdentification and characterization of a hyperthermophilic GH9 cellulase from the Arctic Mid-Ocean Ridge vent fielden_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2019 The Authorsen_US
dc.source.journalPLOS ONE
dc.relation.projectNorges forskningsråd: 270038
dc.relation.projectNorges forskningsråd: 179560
dc.relation.projectNorges forskningsråd: 226247

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