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Adaptation of unbiased stereological methods to study theeffects of feed composition on the morphometrics of the different layers of the digestive tract of juvenile Atlantic cod (Gadus morhua L.)

Bergen Open Research Archive

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dc.contributor.author Pindard, Lars Erik
dc.date.accessioned 2006-06-21T12:37:20Z
dc.date.available 2006-06-21T12:37:20Z
dc.date.issued 2004
dc.identifier.isbn 82-8088-426-2 (electronic veersion)
dc.identifier.uri http://hdl.handle.net/1956/1360
dc.description.abstract Juveniles of Atlantic cod (Gadus morhua L.) were fed food, containing different compositions of macro-nutrients (proteins, fat and carbohydrates). The aims of this study were to evaluate the use of unbiased stereology to find out if there were any differences in estimated weight, volume and/or total surface area and to describe a general digestive tract in these juvenile cod. By employing Technovit 7100 as embedding medium, it was possible to apply a VUR (vertical uniform random) sampling protocol and obtain samples unbiased. Hits from a point-counting grid were used to calculate estimations of volume fraction, and hits from a cycloid counting grid were used for calculating estimations of total surface area of the digestive tract. The estimations showed that there were no significant differences between any of the tested food types in any of the calculated parameters, apart from one, found in the estimated surface ratio of the intestine. This may, however, be due to high variability present in the material. There were no differences between diet groups, regarding thelayers of the digestive tracts whatever diet the fish were given, and the description of the layers in the digestive tract, supports previous findings. en
dc.format.extent 6070070 bytes
dc.format.mimetype application/pdf
dc.language.iso eng en
dc.publisher The University of Bergen en
dc.rights Copyright the author. All rights reserved en
dc.title Adaptation of unbiased stereological methods to study theeffects of feed composition on the morphometrics of the different layers of the digestive tract of juvenile Atlantic cod (Gadus morhua L.) en
dc.type Master thesis
dc.subject.nsi VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 no
dc.rightsHolder The author en
dc.subject.archivecode Mastergrad


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