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Endothelial cells influence the osteogenic potential of bone marrow stromal cells

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dc.contributor.author Xue, Ying
dc.contributor.author Xing, Zhe
dc.contributor.author Hellem, Sølve
dc.contributor.author Arvidson, Kristina Fyrberg
dc.contributor.author Mustafa, Kamal Babikeir Eln
dc.date.accessioned 2011-07-13T13:17:49Z
dc.date.available 2011-07-13T13:17:49Z
dc.date.issued 2009-11-17
dc.identifier.citation BioMedical Engineering OnLine 8(34) en_US
dc.identifier.issn 1475-925X
dc.identifier.uri http://dx.doi.org/10.1186/1475-925X-8-34
dc.identifier.uri http://hdl.handle.net/1956/4834
dc.description.abstract Background: Improved understanding of the interactions between bone cells and endothelial cells involved in osteogenesis should aid the development of new strategies for bone tissue engineering. The aim of the present study was to determine whether direct communication between bone marrow stromal cells (MSC) and human umbilical vein endothelial cells (EC) could influence the osteogenic potential of MSC in osteogenic factor-free medium. Methods: After adding EC to MSC in a direct-contact system, cell viability and morphology were investigated with the WST assay and immnostaining. The effects on osteogenic differentiation of adding EC to MSC was systematically tested by the using Superarray assay and results were confirmed with real-time PCR. Results: Five days after the addition of EC to MSC in a ratio of 1:5 (EC/MSC) significant increases in cell proliferation and cellular bridges between the two cell types were detected, as well as increased mRNA expression of alkaline phosphatase (ALP). This effect was greater than that seen with addition of osteogenic factors such as dexamethasone, ascorbic acid and -glycerophosphate to the culture medium. The expression of transcription factor Runx2 was enhanced in MSC incubated with osteogenic stimulatory medium, but was not influenced by induction with EC. The expression of Collagen type I was not influenced by EC but the cells grown in the osteogenic factorfree medium exhibited higher expression than those cultured with osteogenic stimulatory medium. Conclusion: These results show that co-culturing of EC and MSC for 5 days influences osteogenic differentiation of MSC, an effect that might be independent of Runx2, and enhances the production of ALP by MSC. en_US
dc.language.iso eng en_US
dc.publisher BioMed Central en
dc.rights Copyright 2009 Xue et al; licensee BioMed Central en_US
dc.rights.uri http://creativecommons.org/licenses/by/2.0 en_US
dc.title Endothelial cells influence the osteogenic potential of bone marrow stromal cells en_US
dc.type Journal article en_US
dc.type Peer reviewed en_US
dc.subject.nsi VDP::Medical disciplines: 700::Clinical dentistry disciplines: 830 en_US
dc.rightsHolder Xue et al en_US
dc.type.version publishedVersion en_US


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