dc.contributor.author | Jefimov, Kirill | |
dc.date.accessioned | 2020-12-14T11:48:43Z | |
dc.date.available | 2020-12-14T11:48:43Z | |
dc.date.issued | 2020-12-09 | |
dc.date.submitted | 2020-10-22T12:39:10.359Z | |
dc.identifier | container/c6/2a/35/0e/c62a350e-4553-424d-b6c1-4e2a56a4b92d | |
dc.identifier.isbn | 9788230864371 | |
dc.identifier.isbn | 9788230866764 | |
dc.identifier.uri | https://hdl.handle.net/11250/2719148 | |
dc.description.abstract | The histone modification H3K27ac is a hallmark of active enhancers. However, its role in enhance-specific activity remains obscure. We applied mass spectrometry-based quantitative interaction proteomics to determine proteins that specifically bind H3K27ac. We identified GBAF, a non-canonical GLTSCR1L- and BRD9-containing SWI/SNF chromatin remodeling complex. GBAF was further systematically characterized in terms of protein composition and chromatin localization. A series of ChIP-seq experiments validated the interaction between GLTSCR1L and H3K27ac to be BRD9-dependent. Impairment of the H3K27ac recognition function of GBAF resulted in the dislocation of GLTSCR1L from its preferred binding sites and the genome-wide downregulation, specifically, of enhancer RNA transcription. We show that GBAF binds H3K27ac, and is an enhancer-specific chromatin remodeler involved in the transcriptional and regulatory activity of enhancers. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | The University of Bergen | en_US |
dc.relation.haspart | Paper I: The GBAF chromatin remodeling complex binds H3K27ac and mediates enhancer transcription Kirill Jefimov, Nicolas Alcaraz, Susan L. Kloet, Signe Värv, Siri Aastedatter Sakya, Christian Dalager Vaagenso, Michiel Vermeulen, Rein Aasland, and Robin Andersson bioRxiv 445148. The article is available in the main thesis. The article is also available at: <a href="https://doi.org/10.1101/445148" target="blank">https://doi.org/10.1101/445148</a> | en_US |
dc.rights | Attribution (CC BY). This item's rights statement or license does not apply to the included articles in the thesis. | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | The role of acetylation of histone H3 lysine 27 in enhancer function | en_US |
dc.type | Doctoral thesis | en_US |
dc.date.updated | 2020-10-22T12:39:10.359Z | |
dc.rights.holder | Copyright the Author. | en_US |
dc.contributor.orcid | 0000-0003-0916-4442 | |
dc.description.degree | Doktorgradsavhandling | |
fs.unitcode | 12-60-0 | |