• Cell identity and nucleo-mitochondrial genetic context modulate OXPHOS performance and determine somatic heteroplasmy dynamics 

      Lechuga-Vieco, Ana Victoria; Latorre-Pellicer, Ana; Johnston, Iain; Prota, Gennaro; Gileadi, Uzi; Justo-Méndez, Raquel; Acín-Pérez, Rebeca; Martínez-De-Mena, Raquel; Fernández-Toro, Jose María; Jimenez-Blasco, Daniel; Mora, Alfonso; Nicolás-Ávila, Jose A.; Santiago, Demetrio J.; Priori, Silvia G.; Bolaños, Juan Pedro; Sabio, Guadalupe; Criado, Luis Miguel; Ruíz-Cabello, Jesús; Cerundolo, Vincenzo; Jones, Nick S.; Enríquez, Jose Antonio (Journal article; Peer reviewed, 2020)
      Heteroplasmy, multiple variants of mitochondrial DNA (mtDNA) in the same cytoplasm, may be naturally generated by mutations but is counteracted by a genetic mtDNA bottleneck during oocyte development. Engineered heteroplasmic ...
    • Energetic costs of cellular and therapeutic control of stochastic mitochondrial DNA populations 

      Hoitzing, Hanne; Gammage, Payam; van Haute, Lindsey; Minczuk, Michal; Johnston, Iain; Jones, Nick (Peer reviewed; Journal article, 2019-06-26)
      The dynamics of the cellular proportion of mutant mtDNA molecules is crucial for mitochondrial diseases. Cellular populations of mitochondria are under homeostatic control, but the details of the control mechanisms involved ...
    • HyperTraPS: Inferring probabilistic patterns of trait acquisition in evolutionary and disease progression pathways 

      Greenbury, Sam; Barahona, Mauricio; Johnston, Iain (Peer reviewed; Journal article, 2020)
      The explosion of data throughout the biomedical sciences provides unprecedented opportunities to learn about the dynamics of evolution and disease progression, but harnessing these large and diverse datasets remains ...
    • S100A4 mRNA-protein relationship uncovered by measurement noise reduction 

      Athanasiou, Angelos-Theodoros; Nussbaumer, T; Kummer, Stefan; Hofer, Martin; Johnston, Iain; Staltner, Moritz; Allmer, Daniela M.; Scott, Milcah C.; Vogl, Claus; Fenger, Joelle M.; Modiano, Jaime F.; Walter, Ingrid; Steinborn, Ralf (Journal article; Peer reviewed, 2020)
      Intrinsic biological fluctuation and/or measurement error can obscure the association of gene expression patterns between RNA and protein levels. Appropriate normalization of reverse-transcription quantitative PCR (RT-qPCR) ...