dc.contributor.author | Purohit, Manju | en_US |
dc.contributor.author | Sviland, Lisbeth | en_US |
dc.contributor.author | Wiker, Harald G | en_US |
dc.contributor.author | Mustafa, Tehmina | en_US |
dc.date.accessioned | 2017-08-03T12:54:37Z | |
dc.date.available | 2017-08-03T12:54:37Z | |
dc.date.issued | 2017-04 | |
dc.Published | Purohit MR, Sviland L, Wiker HG, Mustafa T. Rapid and Specific Diagnosis of Extrapulmonary Tuberculosis by Immunostaining of Tissues and Aspirates With Anti-MPT64. Applied immunohistochemistry & molecular morphology (Print). 2017;25(4):282-288 | eng |
dc.identifier.issn | 1541-2016 | |
dc.identifier.uri | https://hdl.handle.net/1956/16201 | |
dc.description.abstract | Background: Extrapulmonary tuberculosis (EPTB) constitutes about 15% to 20% of all cases of tuberculosis (TB). The confirmation of EPTB has always been a challenge to laboratory personnel. We aim to evaluate the diagnostic potential of immunostaining with anti-MPT64 in various EPTB specimens. Materials and Methods: We studied a total of 51 TB cases and 38 non-TB control specimens comprising of fine-needle aspirates and formalin-fixed biopsies. These were investigated using a combination of the Ziehl-Neelsen method, the Lowenstein-Jensen culture, immunostaining with anti-MPT64 and anti-BCG, and nested-polymerase chain reaction (PCR) for IS6110. Results of all the tests were compared using nested-PCR as the gold standard. Results: Diagnostic validation of immunostaining for anti-MPT64 was performed using nested-PCR as the gold standard. The overall sensitivity, specificity, and positive and negative predictive values for immunostaining with anti-MPT64 were 100%, 97%, 97%, and 100%, respectively. Conclusions: Immunostaining using anti-MPT64 is a rapid and sensitive method for establishing an early and specific diagnosis of Mycobacterium tuberculosis infection. The technique is simple to be incorporated into routine pathology laboratories. | en_US |
dc.language.iso | eng | eng |
dc.publisher | Wolters Kluwer | eng |
dc.rights | Attribution CC BY-NC-ND | eng |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | eng |
dc.subject | anti-MPT64 | eng |
dc.subject | immunostaining | eng |
dc.subject | Tuberculosis | eng |
dc.subject | diagnosis | eng |
dc.title | Rapid and Specific Diagnosis of Extrapulmonary Tuberculosis by Immunostaining of Tissues and Aspirates With Anti-MPT64 | en_US |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.date.updated | 2017-05-10T11:53:47Z | |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2016 The Author(s) | |
dc.identifier.doi | https://doi.org/10.1097/pai.0000000000000300 | |
dc.identifier.cristin | 1469365 | |
dc.source.journal | Applied immunohistochemistry & molecular morphology (Print) | |