• norsk
    • English
  • English 
    • norsk
    • English
  • Login
View Item 
  •   Home
  • Faculty of Mathematics and Natural Sciences
  • Department of Informatics
  • Department of Informatics
  • View Item
  •   Home
  • Faculty of Mathematics and Natural Sciences
  • Department of Informatics
  • Department of Informatics
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Protease-dependent fractional mass and peptide properties

Barsnes, Harald; Eidhammer, Ingvar; Cruciani, Véronique; Mikalsen, Svein-Ole
Peer reviewed, Journal article
Thumbnail
View/Open
Published version (629.3Kb)
URI
https://hdl.handle.net/1956/4014
Date
2008-09-22
Metadata
Show full item record
Collections
  • Department of Informatics [541]
Original version
https://doi.org/10.1255/ejms.934
Abstract
Mass spectrometric analyses of peptides mainly rely on cleavage of proteins with proteases that have a defined specificity. The specificities of the proteases imply that there is not a random distribution of amino acids in the peptides. The physico-chemical effects of this distribution have been partly analyzed for tryptic peptides, but to a lesser degree for other proteases. Using all human proteins in Swiss-Prot, the relationships between peptide fractional mass, pI and hydrophobicity were investigated. The distribution of the fractional masses and the average regression lines for the fractional masses were similar, but not identical, for the peptides generated by the proteases trypsin, chymotrypsin and gluC, with the steepest regression line for gluC. The fractional mass regression lines for individual proteins showed up to ±100 ppm in mass difference from the average regression line and the peptides generated showed protease-dependent properties. We here show that the fractional mass and some other properties of the peptides are dependent on the protease used for generating the peptides. With the increasing accuracy of mass spectrometry instruments it is possible to exploit the information embedded in the fractional mass of unknown peaks in peptide mass fingerprint spectra.
Publisher
IM Publications
Copyright
IM Publications
Copyright 2008 IM Publications. All rights reserved.

Contact Us | Send Feedback

Privacy policy
DSpace software copyright © 2002-2019  DuraSpace

Service from  Unit
 

 

Browse

ArchiveCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsDocument TypesJournalsThis CollectionBy Issue DateAuthorsTitlesSubjectsDocument TypesJournals

My Account

Login

Statistics

View Usage Statistics

Contact Us | Send Feedback

Privacy policy
DSpace software copyright © 2002-2019  DuraSpace

Service from  Unit