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dc.contributor.authorBeyene, Demissew Balchaen_US
dc.contributor.authorFranken, Kees Lumcen_US
dc.contributor.authorYamuah, Lawrenceen_US
dc.contributor.authorAseffa, Abrahamen_US
dc.contributor.authorWiker, Harald G.en_US
dc.contributor.authorKolk, Arenden_US
dc.contributor.authorEngers, Howarden_US
dc.contributor.authorKlatser, Paul R.en_US
dc.contributor.authorSviland, Lisbeten_US
dc.date.accessioned2011-03-31T13:00:08Z
dc.date.available2011-03-31T13:00:08Z
dc.date.issued2010eng
dc.PublishedJournal of Infection in Developing Countries 4(2): 96-102en_US
dc.identifier.issn1972-2680
dc.identifier.urihttps://hdl.handle.net/1956/4619
dc.description.abstractBackground: The diagnosis of extra-pulmonary tuberculosis (EPTB) by conventional methods such as culture and microscopy has low sensitivity and requires an invasive procedure. A simple rapid serological test would be of great value. Methodology: Six antigens (ESAT-6, Ag85A, TB10.4, Rv3881c, lipoarabinomannan (LAM) and Ara6-BSA) were tested in an ELISA to detect antigen-specific IgG and IgM antibodies in sera from 54 culture- and histology-confirmed tuberculous lymphadenitis (TBLN) patients as follows: four were HIV seropositive; sera from 25 was smear positive for pulmonary tuberculosis (PTB); 15 were culture- and histology-negative lymphadenitis (non-TBLN) patients; and 22 werehealthy controls (HCs). Results: The sensitivities of the antigens for the detection of IgG in sera of TBLN patients ranged from 4% to 30%. Specificities ranged from 73% to 100% with sera from non-TBLN patients and 91% to 100% with sera from HCs. Sensitivities of the antigens for detection of IgM ranged from 0% to 15% and specificities ranged from 80% to 100% with sera from non-TBLN patients and 91% to 100% with sera from HCs. LAM was the most potent antigen for detection of IgG. When LAM and ESAT-6 were combined, sensitivity increased up to 43% and specificity with non-TBLN was 80% with HC 96%. Conclusions: The study suggests that the combined use of LAM and ESAT-6 for IgG antibody detection in sera from TBLN patients could be a supplement to microscopy of fine-needle aspirate (FNA) to diagnose TBLN among patients suspected of TBLN.en_US
dc.language.isoengeng
dc.publisherOpen Learning on Enteric Pathogenseng
dc.rightsAttribution CC BYeng
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/eng
dc.subjectTuberculous lymphadenitiseng
dc.subjectSerological testeng
dc.subjectAntigenseng
dc.titleSerodiagnosis of tuberculous lymphadenitis using a combination of antigensen_US
dc.typePeer reviewed
dc.typeJournal article
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2010 Beyene et al.
dc.rights.holderBeyene et al.
dc.identifier.cristin344941
dc.subject.nsiVDP::Medical disciplines: 700::Clinical medical disciplines: 750::Communicable diseases: 776eng


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