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dc.contributor.authorLeal, Ernesto Cesar Pinto Jren_US
dc.contributor.authorde Almeida, Patríciaen_US
dc.contributor.authorTomazoni, Shaiane Silvaen_US
dc.contributor.authorde Carvalho, Paulo de Tarso Camilloen_US
dc.contributor.authorLopes-Martins, Rodrigo Alvaro Brandaoen_US
dc.contributor.authorFrigo, Lúcioen_US
dc.contributor.authorJoensen, Jonen_US
dc.contributor.authorJohnson, Mark I.en_US
dc.contributor.authorBjordal, Jan Magnusen_US
dc.description.abstractAim: To evaluate the effects of preventive treatment with low-level laser therapy (LLLT) on progression of dystrophy in mdx mice. Methods: Ten animals were randomly divided into 2 experimental groups treated with superpulsed LLLT (904 nm, 15 mW, 700 Hz, 1 J) or placebo-LLLT at one point overlying the tibialis anterior muscle (bilaterally) 5 times per week for 14 weeks (from 6th to 20th week of age). Morphological changes, creatine kinase (CK) activity and mRNA gene expression were assessed in animals at 20th week of age. Results: Animals treated with LLLT showed very few morphological changes in skeletal muscle, with less atrophy and fibrosis than animals treated with placebo-LLLT. CK was significantly lower (p = 0.0203) in animals treated with LLLT (864.70 U.l−1, SEM 226.10) than placebo (1708.00 U.l−1, SEM 184.60). mRNA gene expression of inflammatory markers was significantly decreased by treatment with LLLT (p<0.05): TNF-α (placebo-control = 0.51 µg/µl [SEM 0.12], - LLLT = 0.048 µg/µl [SEM 0.01]), IL-1β (placebo-control = 2.292 µg/µl [SEM 0.74], - LLLT = 0.12 µg/µl [SEM 0.03]), IL-6 (placebo-control = 3.946 µg/µl [SEM 0.98], - LLLT = 0.854 µg/µl [SEM 0.33]), IL-10 (placebo-control = 1.116 µg/µl [SEM 0.22], - LLLT = 0.352 µg/µl [SEM 0.15]), and COX-2 (placebo-control = 4.984 µg/µl [SEM 1.18], LLLT = 1.470 µg/µl [SEM 0.73]). Conclusion: Irradiation of superpulsed LLLT on successive days five times per week for 14 weeks decreased morphological changes, skeletal muscle damage and inflammation in mdx mice. This indicates that LLLT has potential to decrease progression of Duchenne muscular dystrophy.en_US
dc.rightsAttribution CC BYeng
dc.titleSuperpulsed low-level laser therapy protects skeletal muscle of mdx mice against damage, inflammation and morphological changes delaying dystrophy progressionen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2014 Leal-Junior et al
dc.source.journalPLoS ONE
dc.subject.nsiVDP::Medical sciences: 700::Clinical medical sciences: 750::Physical medicine and rehabilitation: 764eng
dc.subject.nsiVDP::Medical sciences: 700::Clinical medical sciences: 750::Radiology and diagnostic imaging: 763eng
dc.subject.nsiVDP::Medisinske fag: 700::Klinisk medisinske fag: 750::Fysikalsk medisin og rehabilitering: 764nob
dc.subject.nsiVDP::Medisinske fag: 700::Klinisk medisinske fag: 750::Radiologi og bildediagnostikk: 763nob

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