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dc.contributor.authorMunir, Arooj
dc.contributor.authorDøskeland, Anne
dc.contributor.authorAvery, Steven J
dc.contributor.authorFuoco, Tiziana
dc.contributor.authorMohamed Ahmed, Samih
dc.contributor.authorLygre, Henning
dc.contributor.authorFinne-Wistrand, Anna
dc.contributor.authorSloan, Alastair J
dc.contributor.authorWaddington, Rachel J
dc.contributor.authorMustafa, Kamal Babikeir Eln
dc.contributor.authorSuliman, Salwa
dc.PublishedJournal of Tissue Engineering. 2019, 10, 1-16.en_US
dc.description.abstractPoly(L-lactide-co-ε-caprolactone) scaffolds were functionalised by 10 or 20 µg/mL of human demineralised dentine matrix. Release kinetics up to 21 days and their osteogenic potential on human bone marrow stromal cells after 7 and 21 days were studied. A total of 390 proteins were identified by mass spectrometry. Bone regeneration proteins showed initial burst of release. Human bone marrow stromal cells were cultured on scaffolds physisorbed with 20 µg/mL and cultured in basal medium (DDM group) or physisorbed and cultured in osteogenic medium or cultured on non-functionalised scaffolds in osteogenic medium. The human bone marrow stromal cells proliferated less in demineralised dentine matrix group and activated ERK/1/2 after both time points. Cells on DDM group showed highest expression of IL-6 and IL-8 at 7 days and expressed higher collagen type 1 alpha 2, SPP1 and bone morphogenetic protein-2 until 21 days. Extracellular protein revealed higher collagen type 1 and bone morphogenetic protein-2 at 21 days in demineralised dentine matrix group. Cells on DDM group showed signs of mineralisation. The functionalised scaffolds were able to stimulate osteogenic differentiation of human bone marrow stromal cells.en_US
dc.publisherSAGE Publicationsen_US
dc.rightsNavngivelse-Ikkekommersiell 4.0 Internasjonal*
dc.titleEfficacy of copolymer scaffolds delivering human demineralised dentine matrix for bone regenerationen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.rights.holderCopyright The Author(s) 2019en_US
dc.source.journalJournal of Tissue Engineeringen_US

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Navngivelse-Ikkekommersiell 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse-Ikkekommersiell 4.0 Internasjonal