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dc.contributor.authorGrøsvik, Kristin
dc.contributor.authorTesfahun, Almaz Nigatu
dc.contributor.authorMuruzábal-Lecumberri, Izaskun
dc.contributor.authorHaugland, Gyri Teien
dc.contributor.authorLeiros, Ingar
dc.contributor.authorRuoff, Peter
dc.contributor.authorKvaløy, Jan Terje
dc.contributor.authorKnævelsrud, Ingeborg
dc.contributor.authorÅnensen, Hilde
dc.contributor.authorAlexeeva, Marina
dc.contributor.authorSato, Kousuke
dc.contributor.authorMatsuda, Akira
dc.contributor.authorAlseth, Ingrun
dc.contributor.authorKlungland, Arne
dc.contributor.authorBjelland, Svein
dc.PublishedFrontiers in Microbiology. 2020, 11:263 1-17.
dc.description.abstractThe cellular methyl donor S-adenosylmethionine (SAM) and other endo/exogenous agents methylate DNA bases non-enzymatically into products interfering with replication and transcription. An important product is 3-methyladenine (m3A), which in Escherichia coli is removed by m3A-DNA glycosylase I (Tag) and II (AlkA). The tag gene is constitutively expressed, while alkA is induced by sub-lethal concentrations of methylating agents. We previously found that AlkA exhibits activity for the reactive oxygen-induced thymine (T) lesion 5-formyluracil (fU) in vitro. Here, we provide evidence for AlkA involvement in the repair of oxidized bases by showing that the adenine (A) ⋅ T → guanine (G) ⋅ cytosine (C) mutation rate increased 10-fold in E. coli wild-type and alkA– cells exposed to 0.1 mM 5-formyl-2′-deoxyuridine (fdU) compared to a wild-type specific reduction of the mutation rate at 0.2 mM fdU, which correlated with alkA gene induction. G⋅C → A⋅T alleviation occurred without alkA induction (at 0.1 mM fdU), correlating with a much higher AlkA efficiency for fU opposite to G than for that to A. The common keto form of fU is the AlkA substrate. Mispairing with G by ionized fU is favored by its exclusion from the AlkA active site.en_US
dc.publisherFrontiers Mediaen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleThe Escherichia coli alkA Gene Is Activated to Alleviate Mutagenesis by an Oxidized Deoxynucleosideen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.rights.holderCopyright 2020 Grøsvik, Tesfahun, Muruzábal-Lecumberri, Haugland, Leiros,Ruoff, Kvaløy, Knævelsrud, Ånensen, Alexeeva, Sato, Matsuda, Alseth, Klungland and Bjelland.en_US
dc.source.journalFrontiers in Microbiologyen_US
dc.identifier.citationFrontiers in Microbiology. 2020, 11, 263.en_US

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Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal