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dc.contributor.authorDale, Karina
dc.contributor.authorYadetie, Fekadu
dc.contributor.authorMüller, Mette Helen Bjørge
dc.contributor.authorPampanin, Daniela Maria
dc.contributor.authorGilabert, Alejandra
dc.contributor.authorZhang, Xiaokang
dc.contributor.authorTairova, Zhanna
dc.contributor.authorHaarr, Ane
dc.contributor.authorLille-Langøy, Roger
dc.contributor.authorLyche, Jan Ludvig
dc.contributor.authorPorte, Cinta
dc.contributor.authorKarlsen, Odd Andre
dc.contributor.authorGoksøyr, Anders
dc.PublishedAquatic Toxicology. 2020, 227:105590 1-13.
dc.description.abstractThe aim of the present study was to investigate effects of defined mixtures of polycyclic aromatic hydrocarbons (PAHs) and perfluoroalkyl substances (PFASs), at low, environmentally relevant (1× = L), or high (20× = H) doses, on biological responses in Atlantic cod (Gadus morhua). To this end, farmed juvenile cod were exposed at day 0 and day 7 via intraperitoneal (i.p.) injections, in a two-week in vivo experiment. In total, there were 10 groups of fish (n = 21-22): two control groups, four separate exposure groups of PAH and PFAS mixtures (L, H), and four groups combining PAH and PFAS mixtures (L/L, H/L, L/H, H/H). Body burden analyses confirmed a dose-dependent accumulation of PFASs in cod liver and PAH metabolites in bile. The hepatosomatic index (HSI) was significantly reduced for three of the combined PAH/PFAS exposure groups (L-PAH/H-PFAS, H-PAH/L-PFAS, H-PAH/H-PFAS). Analysis of the hepatic proteome identified that pathways related to lipid degradation were significantly affected by PFAS exposure, including upregulation of enzymes in fatty acid degradation pathways, such as fatty acid β-oxidation. The increased abundances of enzymes in lipid catabolic pathways paralleled with decreasing levels of triacylglycerols (TGs) in the H-PFAS exposure group, suggest that PFAS increase lipid catabolism in Atlantic cod. Markers of oxidative stress, including catalase and glutathione S-transferase activities were also induced by PFAS exposure. Only minor and non-significant differences between exposure groups and control were found for cyp1a and acox1 gene expressions, vitellogenin concentrations in plasma, Cyp1a protein synthesis and DNA fragmentation. In summary, our combined proteomics and lipidomics analyses indicate that PFAS may disrupt lipid homeostasis in Atlantic cod.en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleProteomics and lipidomics analyses reveal modulation of lipid metabolism by perfluoroalkyl substances in liver of Atlantic cod (Gadus morhua)en_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.rights.holderCopyright 2020 The Authorsen_US
dc.source.journalAquatic Toxicologyen_US
dc.identifier.citationAquatic Toxicology. 2020, 227, 105590.

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