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dc.contributor.authorOmer, A
dc.contributor.authorAl-sharabi, Niyaz Abdulbaqi
dc.contributor.authorQiu, Y
dc.contributor.authorLi, Y
dc.contributor.authorFujio, Masahito
dc.contributor.authorMustafa, Kamal Babikeir Eln
dc.contributor.authorXing, Zhe
dc.PublishedJournal of Biomedical Materials Research. Part A. 2020, 108 1369-1379.
dc.description.abstractCollagen 1 (COL1) and fibronectin (FN) are extracellular matrix proteins that contribute in cell activity and involve in regulating dental pulp cells (DPCs). The purpose of this study was to investigate the effect of COL1 and FN on the behavior of DPCs. Here, DPCs were grown under three different conditions: COL1 coating, FN coating, and control group without coating. The proliferation and differentiation of DPCs were investigated. DPCs in osteogenic media were able to differentiate into osteoblastic phenotype. The morphological analysis revealed no obvious difference on the shape of cells. Cells had spread well on both coated and noncoated culture plates with slightly more spreading in the coated plates after 24 hr. The MTT analysis did not demonstrate a significant difference at 1 and 3 hr among the groups, but interestingly, the analysis disclosed more cells on the coated plates after longer cultures, which indicated a higher proliferative capacity in response to COL1 and FN. RT-PCR, Western Blotting and mineralization assays did not reveal significant differences between the coated and noncoated surfaces in relation to osteogenic differential potential. Our data suggested that the surface coating of COL1 and FN were able to promote cellular proliferation and the osteogenic differentiation tendency of DPCs was also observed in vitro.en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleBiological responses of dental pulp cells to surfaces modified by collagen 1 and fibronectinen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.rights.holderCopyright 2020 The Authorsen_US
dc.source.journalJournal of Biomedical Materials Research. Part Aen_US
dc.identifier.citationJournal of Biomedical Materials Research. Part A. 2020, 108(6), 1369-1379en_US

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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal