• norsk
    • English
  • English 
    • norsk
    • English
  • Login
View Item 
  •   Home
  • Faculty of Mathematics and Natural Sciences
  • Department of Chemistry
  • Master theses
  • View Item
  •   Home
  • Faculty of Mathematics and Natural Sciences
  • Department of Chemistry
  • Master theses
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Building a Combined Brightfield and Confocal Quantum Diamond Microscope for Imaging of Magnetic Samples

Sti, Olve
Master thesis
Thumbnail
View/Open
master thesis (27.06Mb)
URI
https://hdl.handle.net/11250/2773597
Date
2021-08-02
Metadata
Show full item record
Collections
  • Master theses [71]
Abstract
The imaging of weak magnetic fields has been an important area of both research and tech­nology. It continues to be an area that requires further research due to its potential wealth of applications. One magnetic field imaging technique that has risen in popularity these last few years is called a Quantum Diamond Microscope (QDM). The QDM uses the fluores­cence from negatively charged Nitrogen Vacancy defect centers (NV) in diamond to image magnetic fields due to their usability at room temperature and pressure and high sensitivity to magnetic fields. NV­s are fluoresced with a low­power ∼594 nm laser, the NV­s can also change into a dark, non-­fluorescing, state, where they can remain on a timescale of several seconds. The change of state can be exploited to switch the fluorescence of NV­s off, in a stochastic manner, and it will look as if they are blinking. Combining the NV­s’ blinking together with their sensitivity to magnetic fields allow them to be used as sensors capable of imaging magnetic fields with super resolution. The objective of this Master Thesis is to build a microscope capable of performing Optically Detected Magnetic Resonance (ODMR) on NV­centers. Furthermore, the microscope will be used to carry out Stochastic Optical Reconstruction Microscopy (STORM) to obtain super resolution by exploiting the switch­ing of NV ­to its dark state. The final goal is to combine the two and attempt to do super resolution magnetic field imaging. The main part of this thesis work involve the building of a combined bright-­field and confo­cal microscope. To carry out STORM, a very sensitive photodetector must be implemented. For this purpose, both a Photomultiplier Tube (PMT) and a Si amplified photodetector was tested. Furthermore, the confocal pinhole must be optimized and aligned and a XYZ-­piezo stage was installed for doing Confocal Laser Scanning Microscopy (CLSM). A LabVIEW program was developed to perform CLSM, and other programs in both LabVIEW and MAT­LAB were modified and implemented for the setup. For the confocal imaging, the focused laser spot on the sample was calculated to be 1460±30 nm and the number of NV­s in the focused volumes of the two diamond samples used in this work were estimated to be 2400 NV­s and 12 NV­s. Confocal imaging has been demonstrated and the B field has been mea­sured for 6 different ODMR measurements. Unfortunately it was not possible to demonstrate the blinking of NV­s and further carry out STORM. It was found the PMT and Si amplified photodetector used had too much inherent noise to observe the NV ­blinking. It is suggested that a more sensitive detector like a photon counter detector is necessary to observe this.
Publisher
The University of Bergen
Copyright
Copyright the Author. All rights reserved

Contact Us | Send Feedback

Privacy policy
DSpace software copyright © 2002-2019  DuraSpace

Service from  Unit
 

 

Browse

ArchiveCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsDocument TypesJournalsThis CollectionBy Issue DateAuthorsTitlesSubjectsDocument TypesJournals

My Account

Login

Statistics

View Usage Statistics

Contact Us | Send Feedback

Privacy policy
DSpace software copyright © 2002-2019  DuraSpace

Service from  Unit