dc.contributor.author | Gharbi, Naouel | |
dc.contributor.author | Røise, Dagne | |
dc.contributor.author | Førre, Jorunn-Elise | |
dc.contributor.author | Edson, Amanda Jayne | |
dc.contributor.author | Hushagen, Helena Amalie | |
dc.contributor.author | Tronci, Valentina | |
dc.contributor.author | Frøyset, Ann-Kristin | |
dc.contributor.author | Fladmark, Kari E. | |
dc.date.accessioned | 2022-04-11T13:06:17Z | |
dc.date.available | 2022-04-11T13:06:17Z | |
dc.date.created | 2021-12-02T15:06:03Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 2076-3921 | |
dc.identifier.uri | https://hdl.handle.net/11250/2990970 | |
dc.description.abstract | The eye is continuously under oxidative stress due to high metabolic activity and reactive oxygen species generated by daily light exposure. The redox-sensitive protein DJ-1 has proven to be essential in order to protect retina and retinal pigment epithelium (RPE) from oxidative-stress-induced degeneration. Here, we analyzed the specific role of Müller cell DJ-1 in the adult zebrafish retina by re-establishing Müller-cell-specific DJ-1 expression in a DJ-1 knockout retina. Loss of DJ-1 resulted in an age-dependent retinal degeneration, including loss of cells in the ganglion cell layer, retinal thinning, photoreceptor disorganization and RPE cell dysfunction. The degenerative phenotype induced by the absence of DJ-1 was inhibited by solely expressing DJ-1 in Müller cells. The protective effect was dependent upon the cysteine-106 residue of DJ-1, which has been shown to be an oxidative sensor of DJ-1. In a label-free proteomics analysis of isolated retinas, we identified proteins differentially expressed after DJ-1 knockout, but with restored levels after Müller cell DJ-1 re-insertion. Our data show that Müller cell DJ-1 has a major role in protecting the retina from age-dependent oxidative stress. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | MDPI | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Reintroduction of dj-1 in müller cells inhibits retinal degeneration in the dj-1 deficient retina | en_US |
dc.type | Journal article | en_US |
dc.type | Peer reviewed | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2021 The Author(s) | en_US |
dc.source.articlenumber | 1862 | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.doi | 10.3390/antiox10121862 | |
dc.identifier.cristin | 1963598 | |
dc.source.journal | Antioxidants | en_US |
dc.identifier.citation | Antioxidants. 2021, 10 (12), 1862. | en_US |
dc.source.volume | 10 | en_US |
dc.source.issue | 12 | en_US |