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dc.contributor.authorShanbhag, Siddharth
dc.contributor.authorAl-Sharabi, Niyaz Abdulbaqi Abdulmajid
dc.contributor.authorKampleitner, Carina
dc.contributor.authorMohamed-Ahmed, Samih Salah Eldin Mahgoub
dc.contributor.authorKristoffersen, Einar Klæboe
dc.contributor.authorTangl, Stefan
dc.contributor.authorMustafa, Kamal Babikeir Elnour
dc.contributor.authorGruber, Reinhard
dc.contributor.authorSanz, Mariano
dc.date.accessioned2024-08-01T08:13:31Z
dc.date.available2024-08-01T08:13:31Z
dc.date.created2023-12-05T09:53:05Z
dc.date.issued2024
dc.identifier.issn0905-7161
dc.identifier.urihttps://hdl.handle.net/11250/3143995
dc.description.abstractObjectives Secretomes of mesenchymal stromal cells (MSC) represent a novel strategy for growth-factor delivery for tissue regeneration. The objective of this study was to compare the efficacy of adjunctive use of conditioned media of bone-marrow MSC (MSC-CM) with collagen barrier membranes vs. adjunctive use of conditioned media of leukocyte- and platelet-rich fibrin (PRF-CM), a current growth-factor therapy, for guided bone regeneration (GBR). Methods MSC-CM and PRF-CM prepared from healthy human donors were subjected to proteomic analysis using mass spectrometry and multiplex immunoassay. Collagen membranes functionalized with MSC-CM or PRF-CM were applied on critical-size rat calvaria defects and new bone formation was assessed via three-dimensional (3D) micro-CT analysis of total defect volume (2 and 4 weeks) and 2D histomorphometric analysis of central defect regions (4 weeks). Results While both MSC-CM and PRF-CM revealed several bone-related proteins, differentially expressed proteins, especially extracellular matrix components, were increased in MSC-CM. In rat calvaria defects, micro-CT revealed greater total bone coverage in the MSC-CM group after 2 and 4 weeks. Histologically, both groups showed a combination of regular new bone and ‘hybrid’ new bone, which was formed within the membrane compartment and characterized by incorporation of mineralized collagen fibers. Histomorphometry in central defect sections revealed greater hybrid bone area in the MSC-CM group, while the total new bone area was similar between groups. Conclusion Based on the in vitro and in vivo investigations herein, functionalization of membranes with MSC-CM represents a promising strategy to enhance GBR.en_US
dc.language.isoengen_US
dc.publisherWileyen_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.no*
dc.titleThe use of mesenchymal stromal cell secretome to enhance guided bone regeneration in comparison with leukocyte and platelet-rich fibrinen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2023 The Author(s)en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode2
dc.identifier.doi10.1111/clr.14205
dc.identifier.cristin2208935
dc.source.journalClinical Oral Implants Researchen_US
dc.source.pagenumber141-154en_US
dc.identifier.citationClinical Oral Implants Research. 2024, 35 (2), 141-154.en_US
dc.source.volume35en_US
dc.source.issue2en_US


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Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal