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dc.contributor.authorNaville, Magalien_US
dc.contributor.authorIshibashi, Minakaen_US
dc.contributor.authorFerg, Marcoen_US
dc.contributor.authorBengani, Hemanten_US
dc.contributor.authorRinkwitz, Silkeen_US
dc.contributor.authorKrecsmarik, Monikaen_US
dc.contributor.authorHawkins, Thomas A.en_US
dc.contributor.authorWilson, Stephen W.en_US
dc.contributor.authorManning, Elizabethen_US
dc.contributor.authorChilamakuri, Chandra Sekhar Reddyen_US
dc.contributor.authorWilson, David I.en_US
dc.contributor.authorLouis, Alexandraen_US
dc.contributor.authorRaymond, F. Lucyen_US
dc.contributor.authorRastegar, Sepanden_US
dc.contributor.authorSträhle, Uween_US
dc.contributor.authorLenhard, Borisen_US
dc.contributor.authorBally-Cuif, Laureen_US
dc.contributor.authorvan Heyningen, Veronicaen_US
dc.contributor.authorFitzpatrick, David R.en_US
dc.contributor.authorBecker, Thomas S.en_US
dc.contributor.authorCrollius, Hugues Roesten_US
dc.PublishedNature Communications 2015, 6:6904eng
dc.description.abstractEnhancers can regulate the transcription of genes over long genomic distances. This is thought to lead to selection against genomic rearrangements within such regions that may disrupt this functional linkage. Here we test this concept experimentally using the human X chromosome. We describe a scoring method to identify evolutionary maintenance of linkage between conserved noncoding elements and neighbouring genes. Chromatin marks associated with enhancer function are strongly correlated with this linkage score. We test >1,000 putative enhancers by transgenesis assays in zebrafish to ascertain the identity of the target gene. The majority of active enhancers drive a transgenic expression in a pattern consistent with the known expression of a linked gene. These results show that evolutionary maintenance of linkage is a reliable predictor of an enhancer’s function, and provide new information to discover the genetic basis of diseases caused by the mis-regulation of gene expression.en_US
dc.publisherNature Publishing Group (NPG)eng
dc.rightsAttribution CC BY 4.0eng
dc.subjectBiological scienceseng
dc.titleLong-range evolutionary constraints reveal cis-regulatory interactions on the human X chromosomeen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2015 Rights Managed by Nature Publishing Group
dc.subject.nsiVDP::Medisinske fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk genetikk: 714
dc.subject.nsiVDP::Midical sciences: 700::Basic medical, dental and veterinary sciences: 710::Medical genetics: 714
dc.subject.nsiVDP::Medisinske Fag: 700en_US

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Attribution CC BY 4.0
Except where otherwise noted, this item's license is described as Attribution CC BY 4.0