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dc.contributor.authorSuliman, Salwaen_US
dc.contributor.authorMustafa, Kamal Babikeir Elnen_US
dc.contributor.authorKrueger, Ankeen_US
dc.contributor.authorSteinmüller-Nethl, Dorisen_US
dc.contributor.authorFinne-Wistrand, Annaen_US
dc.contributor.authorOsdal, Terezaen_US
dc.contributor.authorOsman, Amani Hamza Alien_US
dc.contributor.authorSun, Yangen_US
dc.contributor.authorParajuli, Himalayaen_US
dc.contributor.authorWaag, Thiloen_US
dc.contributor.authorNickel, Joachimen_US
dc.contributor.authorJohannessen, Anne Christineen_US
dc.contributor.authorMcCormack, Emmeten_US
dc.contributor.authorCostea, Daniela Elenaen_US
dc.PublishedBiomaterials 2016, 95:11-21eng
dc.description.abstractThis study aimed to evaluate the tumorigenic potential of functionalising poly(LLA-co-CL) scaffolds. The copolymer scaffolds were functionalised with nanodiamonds (nDP) or with nDP and physisorbed BMP-2 (nDP-PHY) to enhance osteoinductivity. Culturing early neoplastic dysplastic keratinocytes (DOKLuc) on nDP modified scaffolds reduced significantly their subsequent sphere formation ability and decreased significantly the cells' proliferation in the supra-basal layers of in vitro 3D oral neoplastic mucosa (3D-OT) when compared to DOKLuc previously cultured on nDP-PHY scaffolds. Using an in vivo non-invasive environmentally-induced oral carcinogenesis model, nDP scaffolds were observed to reduce bioluminescence intensity of tumours formed by DOKLuc + carcinoma associated fibroblasts (CAF). nDP modification was also found to promote differentiation of DOKLuc both in vitro in 3D-OT and in vivo in xenografts formed by DOKLuc alone. The nDP-PHY scaffold had the highest number of invasive tumours formed by DOKLuc + CAF outside the scaffold area compared to the nDP and control scaffolds. In conclusion, in vitro and in vivo results presented here demonstrate that nDP modified copolymer scaffolds are able to decrease the tumorigenic potential of DOKLuc, while confirming concerns for the therapeutic use of BMP-2 for reconstruction of bone defects in oral cancer patients due to its tumour promoting capabilities.en_US
dc.rightsAttribution CC BY-NC-NDeng
dc.subjectBone tissue engineeringeng
dc.subjectOral squamous cell carcinomaeng
dc.titleNanodiamond modified copolymer scaffolds affects tumour progression of early neoplastic oral keratinocytesen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2016 The Author(s)

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