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dc.contributor.authorGjøen, John Espenen_US
dc.contributor.authorJenum, Synneen_US
dc.contributor.authorSivakumaran, Dhanasekaranen_US
dc.contributor.authorMukherjee, Aparnaen_US
dc.contributor.authorMacAden, Raginien_US
dc.contributor.authorKabra, Sushil K.en_US
dc.contributor.authorLodha, Rakeshen_US
dc.contributor.authorOttenhoff, Tom H.M.en_US
dc.contributor.authorHaks, Marielle C.en_US
dc.contributor.authorDoherty, Timothy Marken_US
dc.contributor.authorRitz, Christianen_US
dc.contributor.authorGrewal, Harleenen_US
dc.PublishedGjøen JE, Jenum S, Sivakumaran D, Mukherjee A, MacAden, Kabra SK, Lodha R, Ottenhoff TH, Haks MC, Doherty TM, Ritz C, Grewal H. Novel transcriptional signatures for sputum-independent diagnostics of tuberculosis in children. Scientific Reports. 2017;7:5839eng
dc.description.abstractPediatric tuberculosis (TB) is challenging to diagnose, confirmed by growth of Mycobacterium tuberculosis at best in 40% of cases. The WHO has assigned high priority to the development of non-sputum diagnostic tools. We therefore sought to identify transcriptional signatures in whole blood of Indian children, capable of discriminating intra-thoracic TB disease from other symptomatic illnesses. We investigated the expression of 198 genes in a training set, comprising 47 TB cases (19 definite/28 probable) and 36 asymptomatic household controls, and identified a 7- and a 10-transcript signature, both including NOD2, GBP5, IFITM1/3, KIF1B and TNIP1. The discriminatory abilities of the signatures were evaluated in a test set comprising 24 TB cases (17 definite/7 probable) and 26 symptomatic non-TB cases. In separating TB-cases from symptomatic non-TB cases, both signatures provided an AUC of 0.94 (95%CI, 0.88–1.00), a sensitivity of 91.7% (95%CI, 71.5–98.5) regardless of culture status, and 100% sensitivity for definite TB. The 7-transcript signature provided a specificity of 80.8% (95%CI, 60.0–92.7), and the 10-transcript signature a specificity of 88.5% (95%CI, 68.7–96.9%). Although warranting exploration and validation in other populations, our findings are promising and potentially relevant for future non-sputum based POC diagnostic tools for pediatric TB.en_US
dc.publisherNature Publishing Groupeng
dc.rightsAttribution CC BYeng
dc.titleNovel transcriptional signatures for sputum-independent diagnostics of tuberculosis in childrenen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2017 The Author(s)
dc.source.journalScientific Reports
dc.relation.projectNorges forskningsråd: 248042

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