dc.contributor.author | Bruserud, Øyvind | en_US |
dc.contributor.author | Siddiqui, Huma | en_US |
dc.contributor.author | Marthinussen, Michaela Cuida | en_US |
dc.contributor.author | Chen, Tsute | en_US |
dc.contributor.author | Jonsson, Roland | en_US |
dc.contributor.author | Oftedal, Bergithe Eikeland | en_US |
dc.contributor.author | Olsen, Ingar | en_US |
dc.contributor.author | Husebye, Eystein Sverre | en_US |
dc.contributor.author | Wolff, Anette Bøe | en_US |
dc.date.accessioned | 2018-09-07T10:30:42Z | |
dc.date.available | 2018-09-07T10:30:42Z | |
dc.date.issued | 2018 | |
dc.Published | Bruserud Ø, Siddiqui H, Marthinussen, Chen T, Jonsson R, Oftedal BE, Olsen I, Husebye ES, Wolff. Oral microbiota in autoimmune polyendocrine syndrome type 1. Journal of Oral Microbiology. 2018;10:1442986 | eng |
dc.identifier.issn | 2000-2297 | |
dc.identifier.uri | https://hdl.handle.net/1956/18425 | |
dc.description.abstract | Background: Autoimmune polyendocrine syndrome type-1 (APS-1) is a rare, childhood onset disease caused by mutations in the Autoimmune Regulator gene. The phenotypic expression is highly variable and includes disease manifestations in the oral cavity, including mucocutaneous candidiasis. Increasing evidence suggests a potential role of the skin, oral and gut microbiotas in the pathogenesis of autoimmunity. To date, no information exists regarding the oral microbiota in APS-1. Objective: To assess the bacterial microbiota of whole saliva in APS-1 patients by using high throughput sequencing. Design: Whole unstimulated saliva was collected from 10 APS-1 patients and 17 healthy controls and examined by high throughput sequencing of the hypervariable region V1-V2 of 16S rRNA using the 454 GS Junior system. Metastats (http://cbcb.umd.edu/software/metastats) was used to analyse the pyrosequencing reads. Results: A reduction in the total number of bacterial genera and species was detected in APS-1 compared to healthy controls. The proportion of the major phyla Firmicutes was higher (60% vs 41%, p = 0.002) and Bacteroidetes lower (15% vs 28%, p = 0.007) in APS-1 compared to healthy controls. On the genus level, Streptococcus and Gemella were prevalent in APS-1. Conclusion: Our findings indicate a significantly altered oral microbiota in APS-1. | en_US |
dc.language.iso | eng | eng |
dc.publisher | Taylor & Francis | eng |
dc.rights | Attribution CC BY | eng |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0 | eng |
dc.subject | APS-1 | eng |
dc.subject | whole saliva | eng |
dc.subject | microbiota | eng |
dc.subject | bacteria | eng |
dc.subject | high throughput sequencing | eng |
dc.subject | pyrosequencing | eng |
dc.title | Oral microbiota in autoimmune polyendocrine syndrome type 1 | en_US |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.date.updated | 2018-02-27T07:37:34Z | |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2018 The Author(s) | |
dc.identifier.doi | https://doi.org/10.1080/20002297.2018.1442986 | |
dc.identifier.cristin | 1568917 | |
dc.source.journal | Journal of Oral Microbiology | |