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dc.contributor.authorAasebø, Eliseen_US
dc.contributor.authorHernandez-Valladares, Mariaen_US
dc.contributor.authorSelheim, Frodeen_US
dc.contributor.authorBerven, Frodeen_US
dc.contributor.authorBrenner, Annetteen_US
dc.contributor.authorBruserud, Øysteinen_US
dc.date.accessioned2019-05-29T11:10:18Z
dc.date.available2019-05-29T11:10:18Z
dc.date.issued2018-12-20
dc.PublishedAasebø E, Hernandez-Valladares M, Selheim F, Berven F, Brenner AK, Bruserud Ø. Proteomic Profiling of Primary Human Acute Myeloid Leukemia Cells Does Not Reflect Their Constitutive Release of Soluble Mediators. Proteomes. 2019:7(1):1eng
dc.identifier.issn2227-7382
dc.identifier.urihttps://hdl.handle.net/1956/19807
dc.description.abstractAcute myeloid leukemia (AML) is a heterogeneous disease, and communication between leukemic cells and their neighboring leukemia-supporting normal cells is involved in leukemogenesis. The bone marrow cytokine network is therefore important, and the mediator release profile seems more important than single mediators. It is not known whether the characterization of primary AML cell proteomes reflects the heterogeneity of the broad and dynamic constitutive mediator release profile by these cells. To address this, we compared the intracellular levels of 41 proteins in 19 AML patients with the constitutive extracellular release during in vitro culture, including chemokines, growth factors, proteases, and protease regulators. The constitutive release of most mediators showed a wide variation (up to 2000-fold differences) between patients. Detectable intracellular levels were seen for 10 of 41 mediators, but for most of these 10 mediators we could not detect significant correlations between the constitutive release during in vitro culture and their intracellular levels. Intracellular protein levels in primary human AML cells do not reflect the dynamics, capacity, and variation between patients in constitutive mediator release profiles. Measurements of these profiles thus add complementary information to proteomic detection/quantification regarding the heterogeneity of the AML cell contributions to the bone marrow cytokine network.en_US
dc.language.isoengeng
dc.publisherMDPIeng
dc.rightsAttribution CC BYeng
dc.rights.urihttp://creativecommons.org/licenses/by/4.0eng
dc.subjectAcute myeloid leukemiaeng
dc.subjectcytokineeng
dc.subjectproteaseeng
dc.subjectconstitutive secretioneng
dc.subjectProteomicseng
dc.titleProteomic Profiling of Primary Human Acute Myeloid Leukemia Cells Does Not Reflect Their Constitutive Release of Soluble Mediatorsen_US
dc.typePeer reviewed
dc.typeJournal article
dc.date.updated2019-01-24T10:32:58Z
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2018 The Author(s)
dc.identifier.doihttps://doi.org/10.3390/proteomes7010001
dc.identifier.cristin1646214
dc.source.journalProteomes


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