• norsk
    • English
  • English 
    • norsk
    • English
  • Login
View Item 
  •   Home
  • Faculty of Medicine
  • Department of Clinical Science
  • Department of Clinical Science
  • View Item
  •   Home
  • Faculty of Medicine
  • Department of Clinical Science
  • Department of Clinical Science
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

A Potential Golden Age to Come-Current Tools, Recent Use Cases, and Future Avenues for De Novo Sequencing in Proteomics

Muth, Thilo; Hartkopf, Felix; Vaudel, Marc; Renard, Bernhard Y
Peer reviewed, Journal article
Accepted version
Thumbnail
View/Open
PDF (806.0Kb)
URI
https://hdl.handle.net/1956/20984
Date
2018-07-02
Metadata
Show full item record
Collections
  • Department of Clinical Science [1078]
Original version
https://doi.org/10.1002/pmic.201700150
Abstract
In shotgun proteomics, peptide and protein identification is most commonly conducted using database search engines, the method of choice when reference protein sequences are available. Despite its widespread use the database‐driven approach is limited, mainly because of its static search space. In contrast, de novo sequencing derives peptide sequence information in an unbiased manner, using only the fragment ion information from the tandem mass spectra. In recent years, with the improvements in MS instrumentation, various new methods have been proposed for de novo sequencing. This review article provides an overview of existing de novo sequencing algorithms and software tools ranging from peptide sequencing to sequence‐to‐protein mapping. Various use cases are described for which de novo sequencing was successfully applied. Finally, limitations of current methods are highlighted and new directions are discussed for a wider acceptance of de novo sequencing in the community.
Publisher
Wiley
Journal
Proteomics
Copyright
Copyright 2018 Wiley‐VCH

Contact Us | Send Feedback

Privacy policy
DSpace software copyright © 2002-2019  DuraSpace

Service from  Unit
 

 

Browse

ArchiveCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsDocument TypesJournalsThis CollectionBy Issue DateAuthorsTitlesSubjectsDocument TypesJournals

My Account

Login

Statistics

View Usage Statistics

Contact Us | Send Feedback

Privacy policy
DSpace software copyright © 2002-2019  DuraSpace

Service from  Unit