MassAnalyzer, a program to help find labeled peptides and compare them to their unlabeled counterparts in a SILAC experiment
MetadataShow full item record
Mass spectrometry(MS) have become an increasingly popular analysis method for high throughput experiments on proteins in biology. SILAC(stable isotope labeling by amino acids in cell culture) is a method within MS that uses labels to differentiate between the same protein from two cell cultures(protein pairs) of the same organism. To identify the protein pairs in SILAC MS/MS is used. The current/automatic method for MS/MS uses the most intense peaks from each single MS for the MS/MS analysis. This often result in incomplete pairs, and proteins that have a low abundance gets ignored. We created a program able to identify these pairs, and the researcher can use the data from it as an inclusion list for the MS/MS run. The program should be easy to maintain and use for other MS data analysis.
PublisherThe University of Bergen
Copyright the author. All rights reserved