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dc.contributor.authorValeur, Jørgenen_US
dc.contributor.authorLappalainen, Janien_US
dc.contributor.authorRita, Hannuen_US
dc.contributor.authorLin, Aung Htunen_US
dc.contributor.authorKovanen, Petri T.en_US
dc.contributor.authorBerstad, Arnolden_US
dc.contributor.authorEklund, Kari K.en_US
dc.contributor.authorVaali, Kirsi Kaarinaen_US
dc.PublishedBMC Gastroenterology 9(33)en
dc.description.abstractBackground: We hypothesized that food allergy causes a state of non-specific jejunal dysmotility. This was tested in a mouse model. Methods: Balb/c mice were epicutaneously sensitized with ovalbumin and challenged with 10 intragastric ovalbumin administrations every second day. Smooth muscle contractility of isolated circular jejunal sections was studied in organ bath with increasing concentrations of carbamylcholine chloride (carbachol). Smooth muscle layer thickness and mast cell protease-1 (MMCP-1) positive cell density were assayed histologically. Serum MMCP-1 and immunoglobulins were quantified by ELISA, and mRNA expressions of IFN-γ, IL-4, IL-6 and TGFβ-1 from jejunal and ileal tissue segments were analyzed with quantitative real-time PCR. Results: Ovalbumin-specific serum IgE correlated with jejunal MMCP-1+ cell density. In the allergic mice, higher concentrations of carbachol were required to reach submaximal muscular stimulation, particularly in preparations derived from mice with diarrhoea. Decreased sensitivity to carbachol was associated with increased expression of IL-4 and IL-6 mRNA in jejunum. Smooth muscle layer thickness, as well as mRNA of IFN-γ and TGF-β1 remained unchanged. Conclusion: In this mouse model of food allergy, we demonstrated a decreased response to a muscarinic agonist, and increased levels of proinflammatory IL-6 and Th2-related IL-4, but not Th1- related IFN-γ mRNAs in jejunum. IgE levels in serum correlated with the number of jejunal MMCP- 1+ cells, and predicted diarrhoea. Overall, these changes may reflect a protective mechanism of the gut in food allergy.en_US
dc.publisherBioMed Centraleng
dc.rightsAttribution CC BYeng
dc.titleFood allergy alters jejunal circular muscle contractility and induces local inflammatory cytokine expression in a mouse modelen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2009 Valeur et al; licensee BioMed Central
dc.rights.holderValeur et al
dc.subject.nsiVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Gasteroenterologi: 773nob

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