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dc.contributor.authorBerle, Magnusen_US
dc.contributor.authorKroksveen, Ann Cathrineen_US
dc.contributor.authorHaaland, Øysteinen_US
dc.contributor.authorAye, Thin T.en_US
dc.contributor.authorOpsahl, Jill Anetteen_US
dc.contributor.authorOveland, Eysteinen_US
dc.contributor.authorWester, Knuten_US
dc.contributor.authorUlvik, Rune Johanen_US
dc.contributor.authorHelland, Christian Andreen_US
dc.contributor.authorBerven, Frode S.en_US
dc.PublishedFluids and Barriers of the CNS 2011, 8:19en
dc.description.abstractBackground: The mechanisms behind formation and filling of intracranial arachnoid cysts (AC) are poorly understood. The aim of this study was to evaluate AC fluid by proteomics to gain further knowledge about ACs. Two goals were set: 1) Comparison of AC fluid from individual patients to determine whether or not temporal AC is a homogenous condition; and 2) Evaluate the protein content of a pool of AC fluid from several patients and qualitatively compare this with published protein lists of cerebrospinal fluid (CSF) and plasma. Methods: AC fluid from 15 patients with temporal AC was included in this study. In the AC protein comparison experiment, AC fluid from 14 patients was digested, analyzed by LC-MS/MS using a semi-quantitative label-free approach and the data were compared by principal component analysis (PCA) to gain knowledge of protein homogeneity of AC. In the AC proteome evaluation experiment, AC fluid from 11 patients was pooled, digested, and fractionated by SCX chromatography prior to analysis by LC-MS/MS. Proteins identified were compared to published databases of proteins identified from CSF and plasma. AC fluid proteins not found in these two databases were experimentally searched for in lumbar CSF taken from neurologically-normal patients, by a targeted protein identification approach called MIDAS (Multiple Reaction Monitoring (MRM) initiated detection and sequence analysis). Results: We did not identify systematic trends or grouping of data in the AC protein comparison experiment, implying low variability between individual proteomic profiles of AC. In the AC proteome evaluation experiment, we identified 199 proteins. When compared to previously published lists of proteins identified from CSF and plasma, 15 of the AC proteins had not been reported in either of these datasets. By a targeted protein identification approach, we identified 11 of these 15 proteins in pooled CSF from neurologically-normal patients, demonstrating that the majority of abundant proteins in AC fluid also can be found in CSF. Compared to plasma, as many as 104 proteins in AC were not found in the list of 3017 plasma proteins. Conclusions: Based on the protein content of AC fluid, our data indicate that temporal AC is a homogenous condition, pointing towards a similar AC filling mechanism for the 14 patients examined. Most of the proteins identified in AC fluid have been identified in CSF, indicating high similarity in the qualitative protein content of AC to CSF, whereas this was not the case between AC and plasma. This indicates that AC is filled with a liquid similar to CSF. As far as we know, this is the first proteomics study that explores the AC fluid proteome.en_US
dc.publisherBioMed Centraleng
dc.relation.ispartof<a href="" target="blank">Characterization of arachnoid cysts using clinical chemistry, qualitative and quantitative proteomics</a>eng
dc.rightsAttribution CC BYeng
dc.titleProtein profiling reveals inter-individual protein homogeneity of arachnoid cyst fluid and high qualitative similarity to cerebrospinal fluiden_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2011 Berle et al; licensee BioMed Central Ltd.
dc.subject.nsiVDP::Medical disciplines: 700::Clinical medical disciplines: 750::Oncology: 762eng

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