Department of Biomedicine
Recent Submissions
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Structural and biophysical characterization of transcription factor HNF-1A as a tool to study MODY3 diabetes variants
(Journal article; Peer reviewed, 2022)Hepatocyte nuclear factor 1A (HNF-1A) is a transcription factor expressed in several embryonic and adult tissues, modulating the expression of numerous target genes. Pathogenic variants in the HNF1A gene are known to cause ... -
Mechanical stress shapes the cancer cell response to neddylation inhibition
(Journal article; Peer reviewed, 2022)Background The inhibition of neddylation by the preclinical drug MLN4924 represents a new strategy to combat cancer. However, despite being effective against hematologic malignancies, its success in solid tumors, where ... -
Development and Validation of Arc Nanobodies: New Tools for Probing Arc Dynamics and Function
(Journal article; Peer reviewed, 2022)Activity-regulated cytoskeleton-associated (Arc) protein plays key roles in long-term synaptic plasticity, memory, and cognitive flexibility. However, an integral understanding of Arc mechanisms is lacking. Arc is proposed ... -
Metabolic flux analysis of 3D spheroids reveals significant differences in glucose metabolism from matched 2D cultures of colorectal cancer and pancreatic ductal adenocarcinoma cell lines
(Journal article; Peer reviewed, 2022)Background Most in vitro cancer cell experiments have been performed using 2D models. However, 3D spheroid cultures are increasingly favored for being more representative of in vivo tumor conditions. To overcome the ... -
The Final Maturation State of β-actin Involves N-terminal Acetylation by NAA80, not N-terminal Arginylation by ATE1
(Journal article; Peer reviewed, 2022)Actin is a hallmark protein of the cytoskeleton in eukaryotic cells, affecting a range of cellular functions. Actin dynamics is regulated through a myriad of actin-binding proteins and post-translational modifications. The ... -
Human organotypic airway and lung organoid cells of bronchiolar and alveolar differentiation are permissive to infection by influenza and SARS-CoV-2 respiratory virus
(Journal article; Peer reviewed, 2022)The ongoing coronavirus disease 2019 (COVID-19) pandemic has led to the initiation of unprecedented research efforts to understand the pathogenesis mediated by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). ... -
The styryl benzoic acid derivative DC10 potentiates radiotherapy by targeting the xCT-glutathione axis
(Journal article; Peer reviewed, 2022)Metastatic tumors with moderate radiosensitivity account for most cancer-related deaths, highlighting the limitations of current radiotherapy regimens. The xCT-inhibitor sulfasalazine (SAS) sensitizes cancer cells to ... -
Inhibitory inputs to an inhibitory interneuron: Spontaneous postsynaptic currents and GABA_A receptors of A17 amacrine cells in the rat retina
(Journal article; Peer reviewed, 2022)Amacrine cells constitute a large and heterogeneous group of inhibitory interneurons in the retina. The A17 amacrine plays an important role for visual signalling in the rod pathway microcircuit of the mammalian retina. ... -
Biochemical analysis of novel NAA10 variants suggests distinct pathogenic mechanisms involving impaired protein N‑terminal acetylation
(Journal article; Peer reviewed, 2022)NAA10 is the catalytic subunit of the N-terminal acetyltransferase complex, NatA, which is responsible for N-terminal acetylation of nearly half the human proteome. Since 2011, at least 21 different NAA10 missense variants ... -
The mosaic of AII amacrine cell bodies in rat retina is indistinguishable from a random distribution
(Journal article; Peer reviewed, 2022)The vertebrate retina contains a large number of different types of neurons that can be distinguished by their morphological properties. Assuming that no location should be without a contribution from the circuitry and ... -
Digital reconstruction and quantitative morphometric analysis of bipolar cells in live rat retinal slices
(Journal article; Peer reviewed, 2022)Bipolar cells convey signals from photoreceptors in the outer retina to amacrine and ganglion cells in the inner retina. In mammals, there are typically 10–15 types of cone bipolar cells and one type of rod bipolar cell. ... -
Multiple sclerosis and myelin basic protein: insights into protein disorder and disease
(Journal article; Peer reviewed, 2022)Myelin basic protein (MBP) is an abundant protein in central nervous system (CNS) myelin. MBP has long been studied as a factor in the pathogenesis of the autoimmune neurodegenerative disease multiple sclerosis (MS). MS ... -
Gene-Environment Interactions in Attention-Deficit/Hyperactivity Disorder Symptom Dimensions: The Role of Unhealthy Food Habits
(Journal article; Peer reviewed, 2021)Background: Dietary habits were investigated as environmental risk factors for Attention-Deficit/Hyperactivity Disorder (ADHD). However, no previous studies explored the effects of dietary factors on modifying the role of ... -
Crystal and solution structures reveal oligomerization of individual capsid homology domains of Drosophila Arc
(Journal article; Peer reviewed, 2021)Synaptic plasticity is vital for brain function and memory formation. One of the key proteins in long-term synaptic plasticity and memory is the activity-regulated cytoskeleton-associated protein (Arc). Mammalian Arc forms ... -
Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors
(Journal article; Peer reviewed, 2021)Tumor organoids and patient-derived orthotopic xenografts (PDOXs) are some of the most valuable pre-clinical tools in cancer research. In this protocol, we describe efficient derivation of organoids and PDOX models from ... -
Kidney Segmentation in Renal Magnetic Resonance Imaging - Current Status and Prospects
(Journal article; Peer reviewed, 2021)Magnetic resonance imaging has achieved an increasingly important role in the clinical work-up of renal diseases such chronic kidney disease (CKD). A large panel of parameters have been proposed to diagnose CKD among them ... -
Pharmacokinetics and pharmacodynamics of t-cell bispecifics in the tumour interstitial fluid
(Journal article; Peer reviewed, 2021)The goal of this study is to investigate the pharmacokinetics in plasma and tumour interstitial fluid of two T-cell bispecifics (TCBs) with different binding affinities to the tumour target and to assess the subsequent ... -
Equilibrative Nucleoside Transporters Mediate the Import of Nicotinamide Riboside and Nicotinic Acid Riboside into Human Cells
(Journal article; Peer reviewed, 2021)Nicotinamide riboside (NR), a new form of vitamin B3, is an effective precursor of nicotinamide adenine dinucleotide (NAD+) in human and animal cells. The introduction of NR into the body effectively increases the level ... -
Structural properties and peptide ligand binding of the capsid homology domains of human Arc
(Journal article; Peer reviewed, 2021)The activity-regulated cytoskeleton-associated protein (Arc) is important for synaptic plasticity and the normal function of the brain. Arc interacts with neuronal postsynaptic proteins, but the mechanistic details of its ... -
Healthy kidney segmentation in the dce-mr images using a convolutional neural network and temporal signal characteristics
(Journal article; Peer reviewed, 2021)Quantification of renal perfusion based on dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) requires determination of signal intensity time courses in the region of renal parenchyma. Thus, selection of voxels ...
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