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Melatonin enhances proliferation and modulates differentiation of neural stem cells via autophagy in hyperglycemia

Li, Haoyuan; Zhang, Yanming; Liu, Shangming; Li, Fengpeng; Wang, Benlin; Wang, Jianjie; Cao, Lanfang; Xia, Tongliang; Yao, Qingyu; Chen, Haijun; Zhang, Yulin; Zhu, Xiaodong; Li, Yang; Li, Gang; Wang, Jian; Li, Xingang; Ni, Shilei
Journal article, Peer reviewed
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https://hdl.handle.net/11250/2727458
Utgivelsesdato
2019-01
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  • Department of Biomedicine [596]
  • Registrations from Cristin [5248]
Originalversjon
10.1002/stem.2968
Sammendrag
Dysfunction of neural stem cells (NSCs) has been linked to fetal neuropathy, one of the most devastating complications of gestational diabetes. Several studies have demonstrated that melatonin (Mel) exerted neuroprotective actions in various stresses. However, the role of autophagy and the involvement of Mel in NSCs in hyperglycemia (HG) have not yet been fully established. Here, we found that HG increased autophagy and autophagic flux of NSCs as evidenced by increasing LC3B II/I ratio, Beclin‐1 expression, and autophagosomes. Moreover, Mel enhanced NSCs proliferation and self‐renewal in HG with decreasing autophagy and activated mTOR signaling. Consistently, inhibition of autophagy by 3‐Methyladenine (3‐Ma) could assist Mel effects above, and induction of autophagy by Rapamycin (Rapa) could diminish Mel effects. Remarkably, HG induced premature differentiation of NSCs into neurons (Map2 positive cells) and astrocytes (GFAP positive cells). Furthermore, Mel diminished HG‐induced premature differentiation and assisted NSCs in HG differentiation as that in normal condition. Coincidentally, inhibiting of NSCs autophagy by 3‐Ma assisted Mel to modulate differentiation. However, increasing NSCs autophagy by Rapa disturbed the Mel effects and retarded NSCs differentiation. These findings suggested that Mel supplementation could contribute to mimicking normal NSCs proliferation and differentiation in fetal central nervous system by inhibiting autophagy in the context of gestational diabetes.
Utgiver
AlphaMed Press
Tidsskrift
Stem Cells
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Copyright 2019 AlphaMed Press

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